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Agronomy
Volume 11
Issue 5
10.3390/agronomy11050853
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Article
Peer-Review Record

Integrated Transcriptome and Metabolome Analysis Reveals an Essential Role for Auxin in Hypocotyl Elongation during End-of-Day Far-Red Treatment of Cucurbita moschata (Duch. Ex Lam.)

Agronomy 2021, 11(5), 853; https://doi.org/10.3390/agronomy11050853
by Qi Liu 1,2, Hanqing Zhang 3, Yanhao Mei 1,2, Qi Li 1,2, Yahui Bai 1,2, Huibin Yu 1,2, Xiaodong Xu 1,2, Jianxiang Ma 1, Yongjun Wu 3,* and Zhenchao Yang 1,2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Agronomy 2021, 11(5), 853; https://doi.org/10.3390/agronomy11050853
Submission received: 1 April 2021 / Revised: 22 April 2021 / Accepted: 23 April 2021 / Published: 27 April 2021

Round 1

Reviewer 1 Report

This manuscript was well done described and designed for integrated transcriptome and metabolome analysis in cucurbita moschata.
Analysis of a transcriptome-metabolome introduced in this study was great.

Author Response

  • Thank you very much for your recognition. Best wish for you.

Reviewer 2 Report

The paper is of average  interest.

Some images of  figures (see Fig.1J and 1K) have low  resolution and needed to be improved. Generally, the figures must be presented with the same size and enlargement and the same character size .

Other comments:

-Complete the scientific name classification Cucurbita Moschata.... (L.)?

-Check the punctuation throught the manuscript (see second line pag 2 etc...)

- What is NPA ? Explain

 -Seeds were sown into a 50-hole seedling plate and grown in seedling substrate: please describe what was the"seedling substrate"

- Page 2: After their hypocotyl hooks had emerged!!!? When, how much time, after how much hours/days?

Pag 2: no far-red light or NPA (CK)?? explain (with white light , in dark?)

-M and M: Add the experimental procedure for obtain the dry weight

 - M and M: "The combined extracts........ and filtered for further analysis.: In what consisted the procedure of filtration? Explain

-"Freeze-dried powdered samples (0.1 g) were extracted with 50% methanol, then centrifuged and filtered at 4000 g and 4 ℃" Filtered? see up

-VIP values? explain

-Fig 1. "Above ground part" change these words with other (shoot, shoot system?)

-Legend of Figure 1: add the acronyms with the corresponding description; CK, Control etc... In addition also the significance of the different letters is to be explain in legend and add "Error bars...."

-Fig 2. Delete IAA concentration and report only "IAA (ng/g FW)" in graphic

-Discussion. To the end of first sentence add the reference(s).

-Conclusions. Complete and add that "in presence of  EOD-FR  there were changes in........"

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

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