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Article

Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains

International Potato Center, Avenida La Molina 1895, Lima 15026, Peru
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Academic Editor: David De Koeyer
Agronomy 2021, 11(11), 2315; https://doi.org/10.3390/agronomy11112315
Received: 1 October 2021 / Revised: 4 November 2021 / Accepted: 12 November 2021 / Published: 16 November 2021
(This article belongs to the Special Issue Phenomic and Genomic Tools for the Enhancement of Vegetable Crops)
Potato virus Y (PVY) and Phytophthora infestans (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs. Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY Ryadg were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including full-sibs and half-sibs. Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains. View Full-Text
Keywords: potato; late blight; PVY; quality control; KASP potato; late blight; PVY; quality control; KASP
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MDPI and ACS Style

Kante, M.; Lindqvist-Kreuze, H.; Portal, L.; David, M.; Gastelo, M. Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains. Agronomy 2021, 11, 2315. https://doi.org/10.3390/agronomy11112315

AMA Style

Kante M, Lindqvist-Kreuze H, Portal L, David M, Gastelo M. Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains. Agronomy. 2021; 11(11):2315. https://doi.org/10.3390/agronomy11112315

Chicago/Turabian Style

Kante, Moctar, Hannele Lindqvist-Kreuze, Leticia Portal, Maria David, and Manuel Gastelo. 2021. "Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains" Agronomy 11, no. 11: 2315. https://doi.org/10.3390/agronomy11112315

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