Single-Species Artificial Grasslands Decrease Soil Multifunctionality in a Temperate Steppe on the Qinghai–Tibet Plateau
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Experiment Design
2.3. Soil Sampling and Analysis
2.4. Microbial DNA Extraction, Sequencing, and Data Processing
2.5. Assessing Soil Multifunctionality
2.6. Data Analysis
3. Results
3.1. Impacts of Artificial Grasslands on Soil EMF, Single Functions, Abiotic Factors and Microbial Diversity of the Temperate Steppe
3.2. Relationships between Soil Microbial Diversity and Ecosystem Functionality
3.3. Possible Drivers of Soil EMF
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chen, K.; Zhou, H.; Lu, B.; Wu, Y.; Wang, J.; Zhao, Z.; Li, Y.; Wang, M.; Zhang, Y.; Chen, W.; et al. Single-Species Artificial Grasslands Decrease Soil Multifunctionality in a Temperate Steppe on the Qinghai–Tibet Plateau. Agronomy 2021, 11, 2092. https://doi.org/10.3390/agronomy11112092
Chen K, Zhou H, Lu B, Wu Y, Wang J, Zhao Z, Li Y, Wang M, Zhang Y, Chen W, et al. Single-Species Artificial Grasslands Decrease Soil Multifunctionality in a Temperate Steppe on the Qinghai–Tibet Plateau. Agronomy. 2021; 11(11):2092. https://doi.org/10.3390/agronomy11112092
Chicago/Turabian StyleChen, Kelu, Huakun Zhou, Bingbing Lu, Yang Wu, Jie Wang, Ziwen Zhao, Yuanze Li, Mei Wang, Yue Zhang, Wenjing Chen, and et al. 2021. "Single-Species Artificial Grasslands Decrease Soil Multifunctionality in a Temperate Steppe on the Qinghai–Tibet Plateau" Agronomy 11, no. 11: 2092. https://doi.org/10.3390/agronomy11112092