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Article

Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

1
International Institute of Tropical Agriculture, Ibadan 200001, Nigeria
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The Pan African University Institute of Life and Earth Sciences, University of Ibadan, Ibadan 200132, Nigeria
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Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Hana for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-77900 Olomouc, Czech Republic
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Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, NB E3B 4Z7, Canada
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Department of Crop and Horticultural Sciences, University of Ibadan, Ibadan 200132, Nigeria
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Langridge
Agronomy 2021, 11(10), 1897; https://doi.org/10.3390/agronomy11101897
Received: 12 July 2021 / Revised: 6 September 2021 / Accepted: 15 September 2021 / Published: 22 September 2021
(This article belongs to the Section Crop Breeding and Genetics)
Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop’s genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and interspecific hybridization. View Full-Text
Keywords: flow cytometry; chromosome counting; ploidy level; DArTseq flow cytometry; chromosome counting; ploidy level; DArTseq
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MDPI and ACS Style

Gatarira, C.; Sládeková, L.; Němečková, A.; Šimoníková, D.; Paliwal, R.; Asfaw, A.; Abberton, M.; Badara, G.; Asiedu, R.; Čížková, J.; Hřibová, E.; Doležel, J.; DeKoeyer, D.; Adetimirin, V.; Bhattacharjee, R. Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.). Agronomy 2021, 11, 1897. https://doi.org/10.3390/agronomy11101897

AMA Style

Gatarira C, Sládeková L, Němečková A, Šimoníková D, Paliwal R, Asfaw A, Abberton M, Badara G, Asiedu R, Čížková J, Hřibová E, Doležel J, DeKoeyer D, Adetimirin V, Bhattacharjee R. Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.). Agronomy. 2021; 11(10):1897. https://doi.org/10.3390/agronomy11101897

Chicago/Turabian Style

Gatarira, Cobes, Lucia Sládeková, Alžběta Němečková, Denisa Šimoníková, Rajneesh Paliwal, Asrat Asfaw, Michael Abberton, Gueye Badara, Robert Asiedu, Jana Čížková, Eva Hřibová, Jaroslav Doležel, David DeKoeyer, Victor Adetimirin, and Ranjana Bhattacharjee. 2021. "Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)" Agronomy 11, no. 10: 1897. https://doi.org/10.3390/agronomy11101897

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