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Assessment of the Capacity of Beneficial Bacterial Inoculants to Enhance Canola (Brassica napus L.) Growth under Low Water Activity
Article

Selection of the Root Endophyte Pseudomonas brassicacearum CDVBN10 as Plant Growth Promoter for Brassica napus L. Crops

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Microbiology and Genetics Department, University of Salamanca, 37007 Salamanca, Spain
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Spanish-Portuguese Institute for Agricultural Research (CIALE), Villamayor, 37185 Salamanca, Spain
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Department of Genetics and Microbiology, Faculty of Science, Charles University, 12844 Prague, Czech Republic
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BIOCEV, Institute of Microbiology, the Czech Academy of Sciences, 25242 Vestec, Czech Republic
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Associated R&D Unit, USAL-CSIC (IRNASA), Villamayor, 37185 Salamanca, Spain
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MED—Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research (IIFA), Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal
*
Authors to whom correspondence should be addressed.
Present address: School of Humanities and Social Sciences, University Isabel I, 09003 Burgos, Spain.
Agronomy 2020, 10(11), 1788; https://doi.org/10.3390/agronomy10111788
Received: 5 October 2020 / Revised: 9 November 2020 / Accepted: 12 November 2020 / Published: 15 November 2020
Rapeseed (Brassica napus L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health and the environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers, but efficient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial endophytes, protected from the rhizospheric competitors and extreme environmental conditions, could overcome those problems and successfully promote the crops under field conditions. Here, we present a screening process among rapeseed bacterial endophytes to search for an efficient bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on in vitro, in planta, and in silico tests, we selected the strain Pseudomonas brassicacearum CDVBN10 as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial microbiome; considering that the root microbiome plays an important role in plant fitness and development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis as a positive result. Thus, Pseudomonas brassicacearum CDVBN10 seems to be a good biofertilizer to improve canola crops with no addition of chemical fertilizers; this the first study in which a plant growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves this crop’s yields in field conditions. View Full-Text
Keywords: Pseudomonas; PGPB; bioinoculants; endophytes; bacterial microbiome; culturome; genome sequencing; Brassica napus Pseudomonas; PGPB; bioinoculants; endophytes; bacterial microbiome; culturome; genome sequencing; Brassica napus
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MDPI and ACS Style

Jiménez-Gómez, A.; Saati-Santamaría, Z.; Kostovcik, M.; Rivas, R.; Velázquez, E.; Mateos, P.F.; Menéndez, E.; García-Fraile, P. Selection of the Root Endophyte Pseudomonas brassicacearum CDVBN10 as Plant Growth Promoter for Brassica napus L. Crops. Agronomy 2020, 10, 1788. https://doi.org/10.3390/agronomy10111788

AMA Style

Jiménez-Gómez A, Saati-Santamaría Z, Kostovcik M, Rivas R, Velázquez E, Mateos PF, Menéndez E, García-Fraile P. Selection of the Root Endophyte Pseudomonas brassicacearum CDVBN10 as Plant Growth Promoter for Brassica napus L. Crops. Agronomy. 2020; 10(11):1788. https://doi.org/10.3390/agronomy10111788

Chicago/Turabian Style

Jiménez-Gómez, Alejandro, Zaki Saati-Santamaría, Martin Kostovcik, Raúl Rivas, Encarna Velázquez, Pedro F. Mateos, Esther Menéndez, and Paula García-Fraile. 2020. "Selection of the Root Endophyte Pseudomonas brassicacearum CDVBN10 as Plant Growth Promoter for Brassica napus L. Crops" Agronomy 10, no. 11: 1788. https://doi.org/10.3390/agronomy10111788

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