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Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions

Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10001 Zagreb, Croatia
Faculty of Science, University of Zagreb, Horvatovac102a, 10001 Zagreb, Croatia
Department of Histology and Embryology, School of Medicine, University of Zagreb, Šalata 3, 10001 Zagreb, Croatia
Department of Orthopaedic Surgery, University Hospital, Sveti Duh, 10001 Zagreb, Croatia
Centro de Biomateriales e Ingeniería Tisular, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain
Biomedical Research Networking centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Mariano Esquillor s/n, 50018 Zaragoza, Spain
Author to whom correspondence should be addressed.
Polymers 2017, 9(9), 387;
Received: 21 July 2017 / Revised: 18 August 2017 / Accepted: 21 August 2017 / Published: 23 August 2017
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The extensive need for hard tissue substituent greatly motivates development of suitable allogeneic grafts for therapeutic recreation. Different calcium phosphate phases have been accepted as scaffold’s components with positive influence on osteoinduction and differentiation of human mesenchymal stem cells, in terms of their higher fraction within the graft. Nevertheless, the creation of unlimited nutrients diffusion through newly formed grafts is of great importance. The media flow accomplished by perfusion forces can provide physicochemical, and also, biomechanical stimuli for three-dimensional bone-construct growth. In the present study, the influence of a different scaffold’s composition on the human mesenchymal stem cells (hMSCs) differentiation performed in a U-CUP bioreactor under perfusion conditioning was investigated. The histological and immunohistochemical analysis of cultured bony tissues, and the evaluation of osteogenic genes’ expression indicate that the lower fraction of in situ formed hydroxyapatite in the range of 10–30% within chitosan scaffold could be preferable for bone-construct development. View Full-Text
Keywords: chitosan; hydroxyapatite; hMSC’s; perfusion-bioreactor chitosan; hydroxyapatite; hMSC’s; perfusion-bioreactor

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Rogina, A.; Antunović, M.; Pribolšan, L.; Caput Mihalić, K.; Vukasović, A.; Ivković, A.; Marijanović, I.; Gallego Ferrer, G.; Ivanković, M.; Ivanković, H. Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions. Polymers 2017, 9, 387.

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