Degradation of Albumin on Plasma-Treated Polystyrene by Soft X-ray Exposure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Polystyrene Substrates
2.2. Human Serum Albumin
2.3. Gaseous Plasma Treatment
2.4. Atomic Force Microscopy (AFM)
2.5. X-ray Photoelectron Spectroscopy (XPS)
2.6. Near-Edge X-ray Absorption Fine Structure (NEXAFS)
3. Results and Discussion
3.1. AFM Imaging
3.2. XPS Results
3.3. NEXAFS Analyzes
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
AFM | Atomic force microscopy |
FWHM | Full width at half maximum |
HAS | Human serum albumin |
LUMO | Lowest unoccupied molecular orbital |
NEXAFS | Near-edge X-ray absorption fine structure |
PS | Polystyrene |
RF | Radiofrequency |
UPS | Ultraviolet photoelectron spectroscopy |
UV | Ultraviolet |
VUV | Vacuum ultraviolet radiation |
XANES | X-ray absorption near-edge spectroscopy |
XPS | X-ray photoelectron spectroscopy |
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Sample | C | O | N | F |
---|---|---|---|---|
Untreated PS | 100 | |||
Pure albumin | 66 | 19 | 15 | |
PS + O2 plasma | 73 | 27 | ||
PS + CF4 plasma | 43 | 1 | 56 | |
PS + O2 plasma + albumin prior irradiation | 66 | 18 | 16 | |
PS + O2 plasma + albumin after irradiation | 68 | 20 | 12 | |
PS + CF4 plasma + albumin prior irradiation | 64 | 15 | 11 | 10 |
PS + CF4 plasma + albumin after irradiation | 72 | 12 | 7 | 9 |
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Recek, N.; Primc, G.; Vesel, A.; Mozetic, M.; Avila, J.; Razado-Colambo, I.; Asensio, M.C. Degradation of Albumin on Plasma-Treated Polystyrene by Soft X-ray Exposure. Polymers 2016, 8, 244. https://doi.org/10.3390/polym8070244
Recek N, Primc G, Vesel A, Mozetic M, Avila J, Razado-Colambo I, Asensio MC. Degradation of Albumin on Plasma-Treated Polystyrene by Soft X-ray Exposure. Polymers. 2016; 8(7):244. https://doi.org/10.3390/polym8070244
Chicago/Turabian StyleRecek, Nina, Gregor Primc, Alenka Vesel, Miran Mozetic, José Avila, Ivy Razado-Colambo, and Maria C. Asensio. 2016. "Degradation of Albumin on Plasma-Treated Polystyrene by Soft X-ray Exposure" Polymers 8, no. 7: 244. https://doi.org/10.3390/polym8070244