Design and Preparation of Nano-Lignin Peroxidase (NanoLiP) by Protein Block Copolymerization Approach
1
Department of Chemistry, Anadolu University, 26470 Eskisehir, Turkey
2
Karen Biotechnol Ltd., Anadolu University, Technol Pk, 26470 Eskisehir, Turkey
3
Yunus Emre Vocational School, Department of Medical Services and Techniques, Anadolu University, 26470 Eskisehir, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Katja Loos
Polymers 2016, 8(6), 223; https://doi.org/10.3390/polym8060223
Received: 22 April 2016 / Revised: 9 May 2016 / Accepted: 1 June 2016 / Published: 6 June 2016
(This article belongs to the Special Issue Enzymatic Polymer Synthesis)
This study describes the preparation of nanoprotein particles having lignin peroxidase (LiP) using a photosensitive microemulsion polymerization technique. The protein-based nano block polymer was synthesized by cross-linking of ligninase enzyme with ruthenium-based aminoacid monomers. This type polymerization process brought stability in different reaction conditions, reusability and functionality to the protein-based nano block polymer system when compared the traditional methods. After characterization of the prepared LiP copolymer nanoparticles, enzymatic activity studies of the nanoenzymes were carried out using tetramethylbenzidine (TMB) as the substrate. The parameters such as pH, temperature and initial enzyme concentration that affect the activity, were investigated by using prepared nanoLip particles and compared to free LiP. The reusability of the nano-LiP particles was also investigated and the obtained results showed that the nano-LiP particles exhibited admirable potential as a reusable catalyst.
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Keywords:
nanoenzyme; lignin peroxidase (LiP); photosensitive monomer; ANADOLUCA; tetramethylbenzidine (TMB)
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MDPI and ACS Style
Tay, T.; Köse, E.; Keçili, R.; Say, R. Design and Preparation of Nano-Lignin Peroxidase (NanoLiP) by Protein Block Copolymerization Approach. Polymers 2016, 8, 223. https://doi.org/10.3390/polym8060223
AMA Style
Tay T, Köse E, Keçili R, Say R. Design and Preparation of Nano-Lignin Peroxidase (NanoLiP) by Protein Block Copolymerization Approach. Polymers. 2016; 8(6):223. https://doi.org/10.3390/polym8060223
Chicago/Turabian StyleTay, Turgay; Köse, Ender; Keçili, Rüstem; Say, Rıdvan. 2016. "Design and Preparation of Nano-Lignin Peroxidase (NanoLiP) by Protein Block Copolymerization Approach" Polymers 8, no. 6: 223. https://doi.org/10.3390/polym8060223
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