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Polymers 2016, 8(2), 51;

Conformation and Rheological Properties of Calf-Thymus DNA in Solution

Grenoble Alpes University, Laboratoire Rhéologie et Procédés (LRP), F-38000, Grenoble 38000, France
Biomaterials applications, 6 rue Lesdiguières, Grenoble 38000, France
Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. M. García Barragán, Guadalajara C.P. 44430, Jalisco, Mexico
Author to whom correspondence should be addressed.
Received: 7 January 2016 / Revised: 3 February 2016 / Accepted: 5 February 2016 / Published: 11 February 2016
(This article belongs to the Collection Polyelectrolytes)
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Studies of DNA molecule behavior in aqueous solutions performed through different approaches allow assessment of the solute-solvent interactions and examination of the strong influence of conformation on its physicochemical properties, in the presence of different ionic species and ionic concentrations. Firstly, the conformational behavior of calf-thymus DNA molecules in TE buffer solution is presented as a function of temperature. Secondly, their rheological behavior is discussed, as well as the evidence of the critical concentrations, i.e., the overlap and the entanglement concentrations (C* and Ce, respectively) from steady state flow and oscillatory dynamic shear experiments. The determination of the viscosity in the Newtonian plateau obtained from flow curves η ( ) allows estimation of the intrinsic viscosity and the specific viscosities at zero shear when C[η] < 40. At end, a generalized master curve is obtained from the variation of the specific viscosity as a function of the overlap parameter C[η]. The variation of the exponent s obtained from the power law η~ s for both flow and dynamic results is discussed in terms of Graessley’s analysis. In the semi-dilute regime with entanglements, a dynamic master curve is obtained as a function of DNA concentration (CDNA > 2.0 mg/mL) and temperature (10 °C < T < 40 °C). View Full-Text
Keywords: DNA; hydrodynamic behavior; conformation; electrostatic interactions; temperature DNA; hydrodynamic behavior; conformation; electrostatic interactions; temperature

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Bravo-Anaya, L.M.; Rinaudo, M.; Martínez, F.A.S. Conformation and Rheological Properties of Calf-Thymus DNA in Solution. Polymers 2016, 8, 51.

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