Functionalized Moringa oleifera Gum as pH-Responsive Nanogel for Doxorubicin Delivery: Synthesis, Kinetic Modelling and In Vitro Cytotoxicity Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Preparation of Moringa oleifera Gum Nanogel (MOGN) and DOX-Loaded MOGN (DOX–MOGN)
2.3. Swelling Studies of Moringa oleifera Gum Nanogel (MOGN)
2.4. DOX Loading and Release Studies
2.5. Characterization Studies
2.6. Antitumor Activity Study
2.6.1. Cell Culture
2.6.2. Anti–Tumor Activity Assay
3. Results and Discussion
3.1. Synthesis of Moringa oleifera Gum Nanogel (MOGN)
3.2. All Characterization Studies
3.2.1. FTIR Analysis
3.2.2. SEM Analysis
3.2.3. EDS and Elemental Mapping Analysis
3.2.4. Thermal Analysis
3.3. Swelling Studies of MOGN
3.4. DOX Loading and Release Studies
3.5. In Vitro Cytotoxicity Studies
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Release Models | Equations | Parameters |
---|---|---|
Zero-order | Qt = amount of drug released in time ‘t’ Q0 = initial amount of drug ko = zero-order rate constant | |
First-order | Q = amount of drug remaining in time ‘t’ k1 = first-order rate constant | |
Higuchi | kH = Higuchi dissolution constant | |
Korsmeyer–Peppas | or | Mt/M∞ = fraction of drug released in time ‘t’ kKP = Korsmeyer–Peppas release rate constant n = drug release exponent |
Kinetic Model | Parameters | pHs | ||
---|---|---|---|---|
5.5 | 6.8 | 7.4 | ||
Zero-order | R2 | 0.682 | 0.816 | 0.886 |
ko | 1.06 | 0.902 | 0.254 | |
First-order | R2 | 0.907 | 0.897 | 0.895 |
k1 | 0.019 | 0.007 | 0.001 | |
Higuchi | R2 | 0.808 | 0.916 | 0.956 |
kH | 9.55 | 7.95 | 2.2 | |
Korsmeyer–Peppas | R2 | 0.99 | 0.952 | 0.933 |
kKP | 3.79 | 2.63 | 0.46 | |
n | 0.61 | 0.75 | 1.61 |
Drug Delivery Devices | %L | pH | Pr | Time (h) | References |
---|---|---|---|---|---|
CMXG@AuNPs | 96.4 ± 0.6 | 5.3 6.6 7.4 | 98 ± 4.2 89 ± 1.8 6.67 ± 2.5 | 12 | [4] |
PEG-CMCS-SS-PDPA | ----- | 5.0 | >81.32 | 57 | [5] |
IHC nanoparticles | 79.0 ± 2.1 | 5.0 6.5 7.4 | 67.8 50.2 35 | 100 | [6] |
(Cys-PMO) | 76 | 5.5 7.4 | 56 10 | 48 | [16] |
Chitin-PCLCNGs | 80.0 | 6.0 7.4 | 45.3 36.3 | 72 | [22] |
n-Dxt-p(MBA)-pAA | 86.0 | 5.5 7.4 | 57.0 34.0 | 72 | [23] |
Carboxymethyl-chitosan | 94.7 | 5.0 6.5 7.4 | 95.1 67.2 11.7 | 240 | [25] |
Dextrin | 65–70 | 5.0 6.8 7.4 | 100 94.0 40.0 | 72 | [27] |
Hyaluronate | 63.2 | 5.5 6.8 7.4 | 86.0 60.1 38.5 | 72 | [30] |
Pullulan | 70.0 | 5.0 6.5 7.4 | 46.0 34.2 20.5 | 190 | [31] |
CTNGs | 9.9 | 5.5 7.4 | 70.0 35.0 | 12 | [32] |
Xanthan gum nanogel | --------- | 5.0 6.5 7.4 | 72.1 55.2 38.3 | 72 | [40] |
PEGylated nanogel | 80.0 | 5.3 7.4 | 39.0 13.0 | 50 | [62] |
Chitosan-based nanoparticles | ~75 | 4.5 6.5 7.4 | >80 ~70 ~25 | 48 | [63] |
GO-Phe-CD nanocomposite | 78.7 | 5.3 7.4 | 40 12 | 72 | [64] |
GQDs-PAMAM-β-CD | 61.2 | 5.0 7.4 | 73.87 24.5 | 96 | [65] |
Hyaluronic acid nanogels | 83.33 ± 3.21 | 5.5 7.4 | 61.4 27.2 | 48 | [66] |
CMC/PCL nanofibers | >90 | 5.5 7.4 | ~80 ~35 | ~160 | [67] |
HAHG-B hydrogel | 94 | 5.0 7.4 | ~68 ~32 | 96 | [68] |
DOX-hyd-PEG-FA | --- | 5.0 | 94 | 58 | [69] |
7.4 | 12 | ||||
MOGN | 98.35 | 5.5 6.8 7.4 | 91.92 62.62 12.18 | 72 | Present study |
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Ranote, S.; Musioł, M.; Kowalczuk, M.; Joshi, V.; Chauhan, G.S.; Kumar, R.; Chauhan, S.; Kumar, K. Functionalized Moringa oleifera Gum as pH-Responsive Nanogel for Doxorubicin Delivery: Synthesis, Kinetic Modelling and In Vitro Cytotoxicity Study. Polymers 2022, 14, 4697. https://doi.org/10.3390/polym14214697
Ranote S, Musioł M, Kowalczuk M, Joshi V, Chauhan GS, Kumar R, Chauhan S, Kumar K. Functionalized Moringa oleifera Gum as pH-Responsive Nanogel for Doxorubicin Delivery: Synthesis, Kinetic Modelling and In Vitro Cytotoxicity Study. Polymers. 2022; 14(21):4697. https://doi.org/10.3390/polym14214697
Chicago/Turabian StyleRanote, Sunita, Marta Musioł, Marek Kowalczuk, Veena Joshi, Ghanshyam S. Chauhan, Rakesh Kumar, Sandeep Chauhan, and Kiran Kumar. 2022. "Functionalized Moringa oleifera Gum as pH-Responsive Nanogel for Doxorubicin Delivery: Synthesis, Kinetic Modelling and In Vitro Cytotoxicity Study" Polymers 14, no. 21: 4697. https://doi.org/10.3390/polym14214697