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Volume 14
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10.3390/polym14132667
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Article

Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation

by
Qingbin Guo
1,
Zhengxin Shan
1,
Yanhui Shao
1,
Nifei Wang
1,
Keying Qian
2,
H. Douglas Goff
2,
Qi Wang
3,
Steve W. Cui
2,3 and
Huihuang H. Ding
2,3,*
1
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
2
Department of Food Science, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada
3
Guelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph, ON N1G 5C9, Canada
*
Author to whom correspondence should be addressed.
Polymers 2022, 14(13), 2667; https://doi.org/10.3390/polym14132667
Submission received: 15 May 2022 / Revised: 17 June 2022 / Accepted: 17 June 2022 / Published: 30 June 2022
(This article belongs to the Special Issue Biopolymers: Structure-Function Relationship and Application)
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Abstract

The pectic polysaccharides extracted from flaxseed (Linum usitatissiumum L.) mucilage and kernel were characterized as rhamnogalacturonan-I (RG-I). In this study, the conformational characteristics of RG-I fractions from flaxseed mucilage and kernel were investigated, using a Brookhaven multi-angle light scattering instrument (batch mode) and a high-performance size exclusion chromatography (HPSEC) system coupled with Viscotek tetra-detectors (flow mode). The Mw of flaxseed mucilage RG-I (FM-R) was 285 kDa, and the structure-sensitive parameter (ρ) value of FM-R was calculated as 1.3, suggesting that the FM-R molecule had a star-like conformation. The Mw of flaxseed kernel RG-I (FK-R) was 550 kDa, and the structure-sensitive parameter (ρ) values ranged from 0.90 to 1.21, suggesting a sphere to star-like conformation with relatively higher segment density. The correlation between the primary structure and conformation of RG-I was further discussed to better understand the structure–function relationship, which helps the scale-up applications of pectins in food, pharmaceutical, or cosmetic industries.
Keywords: dietary fibre; flaxseed; pectin; polysaccharide; RG-I; structure–function relationship dietary fibre; flaxseed; pectin; polysaccharide; RG-I; structure–function relationship
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MDPI and ACS Style

Guo, Q.; Shan, Z.; Shao, Y.; Wang, N.; Qian, K.; Goff, H.D.; Wang, Q.; Cui, S.W.; Ding, H.H. Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation. Polymers 2022, 14, 2667. https://doi.org/10.3390/polym14132667

AMA Style

Guo Q, Shan Z, Shao Y, Wang N, Qian K, Goff HD, Wang Q, Cui SW, Ding HH. Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation. Polymers. 2022; 14(13):2667. https://doi.org/10.3390/polym14132667

Chicago/Turabian Style

Guo, Qingbin, Zhengxin Shan, Yanhui Shao, Nifei Wang, Keying Qian, H. Douglas Goff, Qi Wang, Steve W. Cui, and Huihuang H. Ding. 2022. "Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation" Polymers 14, no. 13: 2667. https://doi.org/10.3390/polym14132667

APA Style

Guo, Q., Shan, Z., Shao, Y., Wang, N., Qian, K., Goff, H. D., Wang, Q., Cui, S. W., & Ding, H. H. (2022). Conformational Properties of Flaxseed Rhamnogalacturonan-I and Correlation between Primary Structure and Conformation. Polymers, 14(13), 2667. https://doi.org/10.3390/polym14132667

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