Biomimetic Gradient Scaffolds Containing Hyaluronic Acid and Sr/Zn Folates for Osteochondral Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Porous Support Fabrication
2.3. Preparation of Biomimetic Scaffolds
2.4. Swelling and Degradation Behaviour
2.5. Release of Sr2+, Zn2+ and Folic Acid
2.6. In Vitro Biological Assays
2.6.1. Cell Cultures
2.6.2. Cytotoxicity
2.6.3. Cell Adhesion and Proliferation
2.6.4. Cell Viability
2.6.5. In Vitro Histological Evaluation
2.7. In Vivo Performance
3. Results
3.1. Preparation of the Porous Composite Support Scaffold
3.2. Preparation of Biomimetic Hierarchical Scaffolds
3.3. Swelling/Degradation Behaviour
3.4. Release of Sr2+, Zn2+ and Folic Acid
3.5. In Vitro Biological Evaluation
3.5.1. Cytotoxicity
3.5.2. Cell Viability
3.5.3. Cellular Colonization and Proliferation
3.5.4. In Vitro Histological Analysis
3.6. In Vivo Performance
4. Discussion
4.1. Synthesis of Bioactive Components and Preparation of Porous Scaffolds
4.2. In Vitro Behaviour of Biomimetic Scaffolds
4.3. Biological Response of Biomimetic Scaffolds
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Asensio, G.; Benito-Garzón, L.; Ramírez-Jiménez, R.A.; Guadilla, Y.; Gonzalez-Rubio, J.; Abradelo, C.; Parra, J.; Martín-López, M.R.; Aguilar, M.R.; Vázquez-Lasa, B.; et al. Biomimetic Gradient Scaffolds Containing Hyaluronic Acid and Sr/Zn Folates for Osteochondral Tissue Engineering. Polymers 2022, 14, 12. https://doi.org/10.3390/polym14010012
Asensio G, Benito-Garzón L, Ramírez-Jiménez RA, Guadilla Y, Gonzalez-Rubio J, Abradelo C, Parra J, Martín-López MR, Aguilar MR, Vázquez-Lasa B, et al. Biomimetic Gradient Scaffolds Containing Hyaluronic Acid and Sr/Zn Folates for Osteochondral Tissue Engineering. Polymers. 2022; 14(1):12. https://doi.org/10.3390/polym14010012
Chicago/Turabian StyleAsensio, Gerardo, Lorena Benito-Garzón, Rosa Ana Ramírez-Jiménez, Yasmina Guadilla, Julian Gonzalez-Rubio, Cristina Abradelo, Juan Parra, María Rocío Martín-López, María Rosa Aguilar, Blanca Vázquez-Lasa, and et al. 2022. "Biomimetic Gradient Scaffolds Containing Hyaluronic Acid and Sr/Zn Folates for Osteochondral Tissue Engineering" Polymers 14, no. 1: 12. https://doi.org/10.3390/polym14010012