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Article

Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering

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CNRS UMR 7365 IMoPA - Université de Lorraine, 54500 Vandœuvre-lès-Nancy, France
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Université de Lorraine, CNRS, Arts et Métiers ParisTech, LEM3, F-57000 Metz, France
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Université Reims Champagne-Ardenne, BIOS EA 4691 1, avenue du Maréchal Juin, 51095 Reims, France
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Department of Biomedical Engineering, School of Basic Medical Science, Wuhan University, Wuhan 430000, China
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Université de Lorraine, CNRS, Arts et Métiers ParisTech, LEM3, F-54000 Nancy, France
*
Author to whom correspondence should be addressed.
Polymers 2020, 12(9), 2163; https://doi.org/10.3390/polym12092163
Received: 31 August 2020 / Revised: 18 September 2020 / Accepted: 18 September 2020 / Published: 22 September 2020
(This article belongs to the Special Issue Polymers for Cell Engineering)
(1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering. View Full-Text
Keywords: stromal cells; ligament tissue engineering; braided scaffold; silk; PLCL; biocompatibility stromal cells; ligament tissue engineering; braided scaffold; silk; PLCL; biocompatibility
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MDPI and ACS Style

Liu, X.; Baldit, A.; de Brosses, E.; Velard, F.; Cauchois, G.; Chen, Y.; Wang, X.; de Isla, N.; Laurent, C. Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering. Polymers 2020, 12, 2163. https://doi.org/10.3390/polym12092163

AMA Style

Liu X, Baldit A, de Brosses E, Velard F, Cauchois G, Chen Y, Wang X, de Isla N, Laurent C. Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering. Polymers. 2020; 12(9):2163. https://doi.org/10.3390/polym12092163

Chicago/Turabian Style

Liu, Xing, Adrien Baldit, Emilie de Brosses, Frédéric Velard, Ghislaine Cauchois, Yun Chen, Xiong Wang, Natalia de Isla, and Cédric Laurent. 2020. "Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering" Polymers 12, no. 9: 2163. https://doi.org/10.3390/polym12092163

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