3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Nanoparticles’ Preparation and Characterization
2.2.2. Nanofibers Preparation
2.2.3. Chitosan Aerogels Preparation
2.2.4. Three-Dimensional Scaffolds’ Preparation
2.2.5. FT-IR Analysis
2.2.6. Liquid-Uptake Ability and Swelling Degree
2.2.7. XRD Analysis
2.2.8. SEM and TEM Analysis
2.2.9. Inorganic Matrix Formation and DC-Induced Inorganic Matrix Formation Study
2.2.10. Cytotoxicity Study
3. Results and Discussion
3.1. FT-IR Analysis
3.2. Swelling Properties of the 3D Scaffolds
3.3. XRD Analysis
3.4. Morphology Study of the 3D Scaffolds, and Its Components
3.5. Biomineralization Study
3.6. Cytotoxicity Study of the Prepared Scaffolds
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample | NPs Type, % | Potential, kV |
---|---|---|
CS-PLA-30-HA | - | 30 |
CS-PLA-35-HA | - | 35 |
CS-PLA-30-HA-TiO2 | TiO2, 1.0 | 30 |
CS-PLA-35-HA-TiO2 | TiO2, 1.0 | 35 |
CS-PLA-30-HA-Au | Au, 1.0 | 30 |
CS-PLA-35-HA-Au | Au, 1.0 | 35 |
CS-PLA-30-HA-Pt | Pt, 1.0 | 30 |
CS-PLA-35-HA-Pt | Pt, 1.0 | 35 |
Sample | Standard Biomineralization, % | DC-Induced Biomineralization, % | Ca/P Ratio, - |
---|---|---|---|
CS-PLA-30-HA | 100 | 98 | 1.67 |
CS-PLA-35-HA | 105 | 99 | 1.66 |
CS-PLA-30-HA-TiO2 | 117 | 99 | 1.66 |
CS-PLA-35-HA-TiO2 | 118 | 99 | 1.67 |
CS-PLA-30-HA-Au | 100 | 122 | 1.70 |
CS-PLA-35-HA-Au | 99 | 124 | 1.71 |
CS-PLA-30-HA-Pt | 98 | 116 | 1.69 |
CS-PLA-35-HA-Pt | 98 | 119 | 1.70 |
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Radwan-Pragłowska, J.; Janus, Ł.; Piątkowski, M.; Bogdał, D.; Matysek, D. 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering. Polymers 2020, 12, 792. https://doi.org/10.3390/polym12040792
Radwan-Pragłowska J, Janus Ł, Piątkowski M, Bogdał D, Matysek D. 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering. Polymers. 2020; 12(4):792. https://doi.org/10.3390/polym12040792
Chicago/Turabian StyleRadwan-Pragłowska, Julia, Łukasz Janus, Marek Piątkowski, Dariusz Bogdał, and Dalibor Matysek. 2020. "3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering" Polymers 12, no. 4: 792. https://doi.org/10.3390/polym12040792
APA StyleRadwan-Pragłowska, J., Janus, Ł., Piątkowski, M., Bogdał, D., & Matysek, D. (2020). 3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering. Polymers, 12(4), 792. https://doi.org/10.3390/polym12040792