Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of the N,N′-Dimethylaminoethyl Methacrylate Star and Linear Polymer
2.3. Preparation of Benzophenone Modified Wafers
2.4. Formation of Polymer Layers on the Glass and Silicon Wafers with UV Irradiation
2.5. Quaternization of the Linear and Star PDMAEMA
2.6. Evaluation of Antibacterial Properties
2.7. Characterization Methods
3. Results and Discussion
3.1. Formation of the Layers Made of the Star and Linear Polymers on a Solid Surface
3.2. Physicochemical Characterization of the Obtained PDMAEMA Layers
3.3. Quaternization of the PDMAEMA Nanolayers
3.4. Antibacterial Activity of PDMAEMA and QPDMAEMA Nanolayers
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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PDMAEMA | Mn GPC-MALLS (g/mol) | Mw/Mn |
---|---|---|
L1 | 9000 | 1.09 |
L2 | 13,000 | 1.10 |
L3 | 16,000 | 1.08 |
L4 | 40,000 | 1.12 |
G1 | 320,000 | 2.70 |
G2 | 400,000 | 2.90 |
G3 | 560,000 | 2.33 |
G4 | 1,000,000 | 1.79 |
Sample | C 1s (%) | N 1s (%) | O 1s (%) | Br 3d (%) | C/O |
---|---|---|---|---|---|
SOH | 18.3 | 1.9 | 79.8 | 0.0 | 0.2 |
SBPH | 28.3 | 0.2 | 71.5 | 0.0 | 0.4 |
SL3 | 65.9 | 5.0 | 29.0 | 0.1 | 2.3 |
SG3 | 74.9 | 5.1 | 19.7 | 0.3 | 3.8 |
Layer | Layer Thickness-AFM (nm) | Layer thickness-Ellipsometry (nm) |
---|---|---|
SL1 | 4 | 3 |
SL2 | 6 | 4 |
SL3 | 7 | 6 |
SL4 | 13 | 10 |
SG1 | 31 | 50 |
SG2 | 78 | 70 |
SG3 | 70 | 87 |
SG4 | 120 | 100 |
Nanolayers | Concentration of Quaternized Amino Groups on the Surface (mg/mL/cm2) | Quantity of Quaternized Amino Groups on the cm2 Surface |
---|---|---|
QSL2 | 0.005 | 2.40 × 1016 |
QSL4 | 0.031 | 1.49 × 1017 |
QSG2 | 0.323 | 1.55 × 1018 |
QSG4 | 0.463 | 2.22 × 1018 |
Nanolayer | Number of Bacteria (CFU/mL) 5 min | Growth Inhibition (%) 5 min | Number of Bacteria (CFU/mL) 60 min | Growth Inhibition (%) 60 min | Number of Bacteria (CFU/mL) 24 h | Growth Inhibition (%) 24 h |
---|---|---|---|---|---|---|
SG4 | 1.1 × 106 | 78% | 1.01 × 106 | 80% | 6.1 × 105 | 89% |
QSG4 | 3.3 × 105 | 93% | 2.5 × 106 | 49% | 7.2 × 106 | 0% |
SL4 | 6.2 × 105 | 88% | 6.9 × 106 | 81% | 2.4 × 106 | 62% |
QSL4 | 1.1 × 106 | 79% | 1.6 × 107 | 0% | 1.4 × 106 | 0% |
Control | 5 × 106 | - | 5.2 × 106 | - | 6.2 × 106 | - |
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Teper, P.; Chojniak-Gronek, J.; Hercog, A.; Oleszko-Torbus, N.; Płaza, G.; Kubacki, J.; Balin, K.; Kowalczuk, A.; Mendrek, B. Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity. Polymers 2020, 12, 230. https://doi.org/10.3390/polym12010230
Teper P, Chojniak-Gronek J, Hercog A, Oleszko-Torbus N, Płaza G, Kubacki J, Balin K, Kowalczuk A, Mendrek B. Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity. Polymers. 2020; 12(1):230. https://doi.org/10.3390/polym12010230
Chicago/Turabian StyleTeper, Paulina, Joanna Chojniak-Gronek, Anna Hercog, Natalia Oleszko-Torbus, Grażyna Płaza, Jerzy Kubacki, Katarzyna Balin, Agnieszka Kowalczuk, and Barbara Mendrek. 2020. "Nanolayers of Poly(N,N′-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity" Polymers 12, no. 1: 230. https://doi.org/10.3390/polym12010230