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Article

Antibacterial Activity and Cytotoxicity of Immobilized Glucosamine/Chondroitin Sulfate on Polylactic Acid Films

1
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Nam. T.G.M. 5555, 76001 Zlín, Czech Republic
2
Faculty of Technology, Tomas Bata University in Zlín, Vavreckova 275, 76001 Zlín, Czech Republic
3
Department of Surface Engineering, Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Polymers 2019, 11(7), 1186; https://doi.org/10.3390/polym11071186
Received: 20 June 2019 / Revised: 7 July 2019 / Accepted: 12 July 2019 / Published: 15 July 2019
(This article belongs to the Special Issue Polymer Biointerfaces)
Polylactic acid (PLA) is one of the most produced polymeric materials, due to its exceptional chemical and mechanical properties. Some of them, such as biodegradability and biocompatibility, make them attractive for biomedical applications. Conversely, the major drawback of PLA in the biomedical field is their vulnerability to bacterial contamination. This study focuses on the immobilization of saccharides onto the PLA surface by a multistep approach, with the aim of providing antibacterial features and evaluting the synergistic effect of these saccharides. In this approach, after poly (acrylic acid) (PAA) brushes attached non-covalently to the PLA surface via plasma post-irradiation grafting technique, immobilization of glucosamine (GlcN) and chondroitin sulfate (ChS) to the PAA brushes was carried out. To understand the changes in surface properties, such as chemical composition, surface topography and hydrophilicity, the untreated and treated PLA films were analyzed using various characterization techniques (contact angle, scanning electron microscopy, X-ray photoelectron spectroscopy). In vitro cytotoxicity assays were investigated by the methyl tetrazolium test. The antibacterial activity of the PLA samples was tested against Escherichia coli and Staphylococcus aureus bacteria strains. Plasma-treated films immobilized with ChS and GlcN, separately and in combination, demonstrated bactericidal effect against the both bacteria strains and also the results revealed that the combination has no synergistic effect on antibacterial action. View Full-Text
Keywords: plasma treatment; surface modification; saccharide immobilization; antibacterial activity; count-plate method; cytotoxicity; chondroitin sulfate plasma treatment; surface modification; saccharide immobilization; antibacterial activity; count-plate method; cytotoxicity; chondroitin sulfate
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MDPI and ACS Style

Karakurt, I.; Ozaltin, K.; Vesela, D.; Lehocky, M.; Humpolíček, P.; Mozetič, M. Antibacterial Activity and Cytotoxicity of Immobilized Glucosamine/Chondroitin Sulfate on Polylactic Acid Films. Polymers 2019, 11, 1186. https://doi.org/10.3390/polym11071186

AMA Style

Karakurt I, Ozaltin K, Vesela D, Lehocky M, Humpolíček P, Mozetič M. Antibacterial Activity and Cytotoxicity of Immobilized Glucosamine/Chondroitin Sulfate on Polylactic Acid Films. Polymers. 2019; 11(7):1186. https://doi.org/10.3390/polym11071186

Chicago/Turabian Style

Karakurt, Ilkay, Kadir Ozaltin, Daniela Vesela, Marian Lehocky, Petr Humpolíček, and Miran Mozetič. 2019. "Antibacterial Activity and Cytotoxicity of Immobilized Glucosamine/Chondroitin Sulfate on Polylactic Acid Films" Polymers 11, no. 7: 1186. https://doi.org/10.3390/polym11071186

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