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Polymers 2019, 11(3), 451; https://doi.org/10.3390/polym11030451

Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide

1
Departamento de Nanociencias y Nanotecnología. Centro de Investigación y de Estudios Avanzados del IPN. Av. IPN 2508, la laguna Ticomán, Ciudad de México 07360, Mexico
2
Instituto Tecnológico Superior de Ciudad Hidalgo. Av. Ing. Carlos Rojas Gutiérrez 2120, fracc. Valle de la herradura, Michoacán 61100, Mexico
3
CONACYT-Facultad de Medicina y Cirugía Universidad Autónoma Benito Juárez de Oaxaca, Ex Hacienda de Aguilera S/N, Carretera a San Felipe del Agua S/N, Oaxaca 68020, Mexico
4
Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN 2508, la laguna Ticomán, Ciudad de México 07360, Mexico
5
Departamento de investigación y posgrado en alimentos, Facultad de Química, Universidad Autónoma de Querétaro, Centro universitario, Santiago de Querétaro, Querétaro 76010, Mexico
*
Authors to whom correspondence should be addressed.
Received: 19 December 2018 / Revised: 12 February 2019 / Accepted: 28 February 2019 / Published: 9 March 2019
(This article belongs to the Special Issue Polymeric Materials for Tissue Engineering)
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Abstract

In this study, a coating from electrospun silk fibroin was performed with the aim to modify the surface of breast implants. We evaluated the effect of fibroin on polymeric matrices of poly (ethylene oxide) (PEO) to enhance cell viability, adhesion, and proliferation of HaCaT human keratinocytes to enhance the healing process on breast prosthesis implantation. We electrospun six blends of fibroin and PEO at different concentrations. These scaffolds were characterized by scanning electron microscopy, contact angle measurements, ATR-FTIR spectroscopy, and X-ray diffraction. We obtained diverse network conformations at different combinations to examine the regulation of cell adhesion and proliferation by modifying the microstructure of the matrix to be applied as a potential scaffold for coating breast implants. The key contribution of this work is the solution it provides to enhance the healing process on prosthesis implantation considering that the use of these PEO–fibroin scaffolds reduced (p < 0.05) the amount of pyknotic nuclei. Therefore, viability of HaCaT human keratinocytes on PEO–fibroin matrices was significantly improved (p < 0.001). These findings provide a rational strategy to coat breast implants improving biocompatibility. View Full-Text
Keywords: silk fibroin; surface modification; electrospinning; ultrafiltration silk fibroin; surface modification; electrospinning; ultrafiltration
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Carrasco-Torres, G.; Valdés-Madrigal, M.A.; Vásquez-Garzón, V.R.; Baltiérrez-Hoyos, R.; De la Cruz-Burelo, E.; Román-Doval, R.; Valencia-Lazcano, A.A. Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide. Polymers 2019, 11, 451.

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