Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide
AbstractIn this study, a coating from electrospun silk fibroin was performed with the aim to modify the surface of breast implants. We evaluated the effect of fibroin on polymeric matrices of poly (ethylene oxide) (PEO) to enhance cell viability, adhesion, and proliferation of HaCaT human keratinocytes to enhance the healing process on breast prosthesis implantation. We electrospun six blends of fibroin and PEO at different concentrations. These scaffolds were characterized by scanning electron microscopy, contact angle measurements, ATR-FTIR spectroscopy, and X-ray diffraction. We obtained diverse network conformations at different combinations to examine the regulation of cell adhesion and proliferation by modifying the microstructure of the matrix to be applied as a potential scaffold for coating breast implants. The key contribution of this work is the solution it provides to enhance the healing process on prosthesis implantation considering that the use of these PEO–fibroin scaffolds reduced (p < 0.05) the amount of pyknotic nuclei. Therefore, viability of HaCaT human keratinocytes on PEO–fibroin matrices was significantly improved (p < 0.001). These findings provide a rational strategy to coat breast implants improving biocompatibility. View Full-Text
Share & Cite This Article
Carrasco-Torres, G.; Valdés-Madrigal, M.A.; Vásquez-Garzón, V.R.; Baltiérrez-Hoyos, R.; De la Cruz-Burelo, E.; Román-Doval, R.; Valencia-Lazcano, A.A. Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide. Polymers 2019, 11, 451.
Carrasco-Torres G, Valdés-Madrigal MA, Vásquez-Garzón VR, Baltiérrez-Hoyos R, De la Cruz-Burelo E, Román-Doval R, Valencia-Lazcano AA. Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide. Polymers. 2019; 11(3):451.Chicago/Turabian Style
Carrasco-Torres, Gabriela; Valdés-Madrigal, Manuel A.; Vásquez-Garzón, Verónica R.; Baltiérrez-Hoyos, Rafael; De la Cruz-Burelo, Eduard; Román-Doval, Ramón; Valencia-Lazcano, Anaí A. 2019. "Effect of Silk Fibroin on Cell Viability in Electrospun Scaffolds of Polyethylene Oxide." Polymers 11, no. 3: 451.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.