Next Article in Journal
Balanced Viscoelastic Properties of Pressure Sensitive Adhesives Made with Thermoplastic Polyurethanes Blends
Previous Article in Journal
Macromolecular Brushes Based on Poly(L-Lactide) and Poly(ε-Caprolactone) Single and Double Macromonomers via ROMP. Synthesis, Characterization and Thermal Properties
Previous Article in Special Issue
Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
Open AccessArticle

Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media

1
Department of Chemistry and Materials Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan
2
Collaborative Research Center of Engineering, Medicine, and Pharmacology, ORDIST, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan
*
Authors to whom correspondence should be addressed.
Polymers 2019, 11(10), 1607; https://doi.org/10.3390/polym11101607
Received: 13 July 2019 / Revised: 20 September 2019 / Accepted: 28 September 2019 / Published: 2 October 2019
(This article belongs to the Special Issue Biomimetic Polymer-Based Matrices for Regenerative Medicine)
Application of Na+-responsive DNA quadruplex hydrogels, which utilize G-quadruplexes as crosslinking points of poly(ethylene glycol) (PEG) network as cell culture substrate, has been examined. PEG-oligodeoxynucleotide (ODN) conjugate, in which four deoxyguanosine (dG4) residues are tethered to both ends of PEG, was prepared by modified high-efficiency liquid phase (HELP) synthesis of oligonucleotides and used as the macromonomer. When mixed with equal volume of cell culture media, the solution of PEG-ODN turned into stiff hydrogel (G-quadruplex hydrogel) as the result of G-quadruplex formation by the dG4 segments in the presence of Na+. PEG-ODN itself did not show cytotoxicity and the resulting hydrogel was stable enough under cell culture conditions. However, L929 fibroblast cells cultured in G-quadruplex hydrogel remained spherical for a week, yet alive, without proliferation. The cells gradually sedimented through the gel day by day, probably due to the reversible nature of G-quadruplex formation and the resulting slow rearrangement of the macromonomers. Once they reached the bottom glass surface, the cells started to spread and proliferate. View Full-Text
Keywords: hydrogels; DNA; G-quadruplexes; polyethylene glycol; cell culture hydrogels; DNA; G-quadruplexes; polyethylene glycol; cell culture
Show Figures

Graphical abstract

MDPI and ACS Style

Tanaka, S.; Yukami, S.; Hachiro, Y.; Ohya, Y.; Kuzuya, A. Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media. Polymers 2019, 11, 1607.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop