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Article

Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation

1
Department of Health Sciences and Technology, GAIHST, Gachon University, 155 Gaetbeol-ro, Yeonsu-ku, Incheon 21999, Korea
2
Department of Molecular Medicine, College of Medicine, Gachon University, 155 Gaetbeol-ro, Yeonsu-ku, Incheon 21999, Korea
3
Soonchunhyang Institute of Medi-bio Science, Soonchunhyang University, Cheonan-si 31151, Korea
4
Department of Thoracic and Cardiovascular Surgery, Gil Medical Center, Gachon University College of Medicine, 21, Namdong-daero 774 Beon-gil, Namdong-gu, Incheon 21565, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Polymers 2019, 11(4), 643; https://doi.org/10.3390/polym11040643
Received: 7 March 2019 / Revised: 30 March 2019 / Accepted: 4 April 2019 / Published: 9 April 2019
(This article belongs to the Special Issue Biomimetic Polymer-Based Matrices for Regenerative Medicine)
Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µm are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation. View Full-Text
Keywords: electrospinning; vascular smooth muscle; vascular scaffold; infiltration; optimization electrospinning; vascular smooth muscle; vascular scaffold; infiltration; optimization
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MDPI and ACS Style

Han, D.G.; Ahn, C.B.; Lee, J.-H.; Hwang, Y.; Kim, J.H.; Park, K.Y.; Lee, J.W.; Son, K.H. Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation. Polymers 2019, 11, 643. https://doi.org/10.3390/polym11040643

AMA Style

Han DG, Ahn CB, Lee J-H, Hwang Y, Kim JH, Park KY, Lee JW, Son KH. Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation. Polymers. 2019; 11(4):643. https://doi.org/10.3390/polym11040643

Chicago/Turabian Style

Han, Dae G., Chi B. Ahn, Ji-Hyun Lee, Yongsung Hwang, Joo H. Kim, Kook Y. Park, Jin W. Lee, and Kuk H. Son. 2019. "Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation" Polymers 11, no. 4: 643. https://doi.org/10.3390/polym11040643

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