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Polymers 2018, 10(3), 292; https://doi.org/10.3390/polym10030292

Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp.

Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, 1 Tongwei Road, Nanjing 210095, China
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Received: 16 January 2018 / Revised: 2 March 2018 / Accepted: 4 March 2018 / Published: 9 March 2018
(This article belongs to the Special Issue Polysaccharides)
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Abstract

The arctic strain of Chlorella sp. (Chlorella-Arc) exists in the coldest and driest arctic ecosystems, and it is a new resource of active polysaccharides. The extraction of crude polysaccharide from Chlorella-Arc was optimized using the response surface methodology. A crude polysaccharide yield of approximately 9.62 ± 0.11% dry weight was obtained under these optimized conditions. Three fractions (P-I, P-II, and P-III) were present after purification by 2-diethylaminoethanol Sepharose Fast Flow and Sephadex G-100 chromatography. The P-IIa fraction demonstrated significant antioxidant activities. Moreover, P-IIa was an α- and β-type heteropolysaccharide with a pyran group and contained variable amounts of rhamnose, arabinose, glucose, and galactose based on fourier-transform infrared spectroscopy, high-performance liquid chromatography, and 1H and 13C nuclear magnetic resonance imaging. Production of high amounts of polysaccharide may allow further exploration of the microalgae Chlorella-Arc as a natural antioxidant. View Full-Text
Keywords: arctic Chlorella sp.; polysaccharide; antioxidant activity; preliminary structural characterization arctic Chlorella sp.; polysaccharide; antioxidant activity; preliminary structural characterization
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Song, H.; He, M.; Gu, C.; Wei, D.; Liang, Y.; Yan, J.; Wang, C. Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp.. Polymers 2018, 10, 292.

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