Next Article in Journal
Compressive Behavior of Aluminum Microfibers Reinforced Semi-Rigid Polyurethane Foams
Next Article in Special Issue
Effect of the Surface Hydrophobicity Degree on the In Vitro Release of Polar and Non-Polar Drugs from Polyelectrolyte Matrix Tablets
Previous Article in Journal
Degradation of Polyacrylate in the Outdoor Agricultural Soil Measured by FTIR-PAS and LIBS
Previous Article in Special Issue
Conformation Study of Dual Stimuli-Responsive Core-Shell Diblock Polymer Brushes
Article Menu

Export Article

Open AccessArticle
Polymers 2018, 10(12), 1297; https://doi.org/10.3390/polym10121297

Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length

1
Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., P.O. Box 278, Irkutsk 664033, Russia
2
Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu, India
*
Author to whom correspondence should be addressed.
Received: 22 October 2018 / Revised: 16 November 2018 / Accepted: 21 November 2018 / Published: 23 November 2018
Full-Text   |   PDF [7748 KB, uploaded 23 November 2018]   |  
  |   Review Reports

Abstract

Amine containing polymers are extensively studied as special carriers for short-chain RNA (13–25 nucleotides), which are applied as gene silencing agents in gene therapy of various diseases including cancer. Elaboration of the oligonucleotide carriers requires knowledge about peculiarities of the oligonucleotide–polymeric amine interaction. The critical length of the interacting chains is an important parameter which allows us to design sophisticated constructions containing oligonucleotide binding segments, solubilizing, protective and aiming parts. We studied interactions of (TCAG)n, n = 1–6 DNA oligonucleotides with polyethylenimine and poly(N-(3-((3-(dimethylamino)propyl)(methyl)amino)propyl)-N-methylacrylamide). The critical length for oligonucleotides in interaction with polymeric amines is 8–12 units and complexation at these length can be accompanied by “all-or-nothing” effects. New dimethylacrylamide based polymers with grafted polyamine chains were obtained and studied in complexation with DNA and RNA oligonucleotides. The most effective interaction and transfection activity into A549 cancer cells and silencing efficiency against vascular endothelial growth factor (VEGF) was found for a sample with average number of nitrogens in polyamine chain equal to 27, i.e., for a sample in which all grafted chains are longer than the critical length for polymeric amine–oligonucleotide complexation. View Full-Text
Keywords: polymeric amines; oligonucleotides; critical length; grafted polyamines; gene delivery polymeric amines; oligonucleotides; critical length; grafted polyamines; gene delivery
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Annenkov, V.V.; Krishnan, U.M.; Pal’shin, V.A.; Zelinskiy, S.N.; Kandasamy, G.; Danilovtseva, E.N. Design of Oligonucleotide Carriers: Importance of Polyamine Chain Length. Polymers 2018, 10, 1297.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top