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Polymers 2018, 10(10), 1080;

Biodegradable Gene Carriers Containing Rigid Aromatic Linkage with Enhanced DNA Binding and Cell Uptake

Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, China
Authors to whom correspondence should be addressed.
Received: 31 August 2018 / Revised: 21 September 2018 / Accepted: 27 September 2018 / Published: 29 September 2018
(This article belongs to the Special Issue Polymers in Gene Delivery)
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The linking and modification of low molecular weight cationic polymers (oligomers) has become an attracted strategy to construct non-viral gene carriers with good transfection efficiency and much reduced cytotoxicity. In this study, PEI 600 Da was linked by biodegradable bridges containing rigid aromatic rings. The introduction of aromatic rings enhanced the DNA-binding ability of the target polymers and also improved the stability of the formed polymer/DNA complexes. The biodegradable property and resulted DNA release were verified by enzyme stimulated gel electrophoresis experiment. These materials have lower molecular weights compared to PEI 25 kDa, but exhibited higher transfection efficiency, especially in the presence of serum. Flow cytometry and confocal laser scanning microscopy results indicate that the polymers with aromatic rings could induce higher cellular uptake. This strategy for the construction of non-viral gene vectors may be applied as an efficient and promising method for gene delivery. View Full-Text
Keywords: non-viral vectors; gene delivery; polyethylenimine; structure–activity relationship non-viral vectors; gene delivery; polyethylenimine; structure–activity relationship

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Zhang, J.-H.; Yang, H.-Z.; Zhang, J.; Liu, Y.-H.; He, X.; Xiao, Y.-P.; Yu, X.-Q. Biodegradable Gene Carriers Containing Rigid Aromatic Linkage with Enhanced DNA Binding and Cell Uptake. Polymers 2018, 10, 1080.

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