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Polymers 2018, 10(10), 1048; https://doi.org/10.3390/polym10101048

Fibrin Hydrogels for Endothelialized Liver Tissue Engineering with a Predesigned Vascular Network

1
Department of Tissue Engineering, Center of 3D Printing & Organ Manufacturing, School of Fundamental Sciences, China Medical University (CMU), No. 77 Puhe Road, Shenyang North New Area, Shenyang 110122, China
2
Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China
3
Tianjin Mifang Science & Technology Ltd., Wuqing 301701, China
*
Author to whom correspondence should be addressed.
Received: 27 July 2018 / Revised: 4 September 2018 / Accepted: 18 September 2018 / Published: 20 September 2018
(This article belongs to the Special Issue Hydrogels and Gels for Biomedical and Sustainable Applications)
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Abstract

The design and manufacture of a branched vascular network is essential for bioartificial organ implantation, which provides nutrients and removes metabolites for multi-cellular tissues. In the present study, we present a technology to manufacture endothelialized liver tissues using a fibrin hydrogel and a rotational combined mold. Both hepatocytes and adipose-derived stem cells (ADSCs) encapsulated in a fibrin hydrogel were assembled into a spindle construct with a predesigned multi-branched vascular network. An external overcoat of poly(dl-lactic-co-glycolic acid) was used to increase the mechanical properties of the construct as well as to act as an impervious and isolating membrane around the construct. Cell survivability reached 100% in the construct after 6 days of in vitro culture. ADSCs in the spindle construct were engaged into endothelial cells/tissues using a cocktail growth factor engagement approach. Mechanical property comparison and permeability evaluation tests all indicated that this was a viable complex organ containing more than two heterogeneous tissue types and a functional vascular network. It is, therefore, the first time an implantable bioartificial liver, i.e., endothelialized liver tissue, along with a hierarchical vascular network, has been created. View Full-Text
Keywords: fibrin hydrogel; artificial livers; rotational combined mold; branched vascular network; adipose-derived stem cells (ADSCs) fibrin hydrogel; artificial livers; rotational combined mold; branched vascular network; adipose-derived stem cells (ADSCs)
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Wang, X.; Liu, C. Fibrin Hydrogels for Endothelialized Liver Tissue Engineering with a Predesigned Vascular Network. Polymers 2018, 10, 1048.

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