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Polymers 2018, 10(1), 97;

Synthesis of Molecularly Imprinted Cryogels to Deplete Abundant Proteins from Bovine Serum

School of Chemistry & Chemical Engineering, Yangzhou University, 180 Siwangting RD, Yangzhou 225002, China
Author to whom correspondence should be addressed.
Received: 30 November 2017 / Revised: 15 January 2018 / Accepted: 16 January 2018 / Published: 20 January 2018
(This article belongs to the Special Issue Molecularly Imprinted Polymers)
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Molecularly imprinted polyacrylamide cryogels were synthesized with pending templates (bovine serums of different concentrations). As the serum concentrations increased in the monomer solutions, the resulting cryogels could adsorb and deplete more proteins from serum samples. Due to the addition of vinyltriethoxysilane (VTEOS) in the prepolymerizing solutions, the polymers came as organic–inorganic hybrid materials. It endued the silica-modified amphoteric polyacrylamide cryogels with improved mechanical strengths. Scanning electron micrography (SEM), Infrared (IR) spectrometry, thermogravimetry-differential thermal analysis (TG-DTA), and X-ray photoelectron spectroscopy (XPS) were carried out to characterize these macroporous polymers. Amphoteric cryogels proved to be favorable materials recognizing and binding proteins. When used as liquid chromatography stationary phases, they were capable of simultaneously adsorbing various serum proteins. Electrophoresis showed that abundant proteins were gradually depleted by the cryogels prepared from increased ratios of bovine serums in the monomer solutions. As abundant proteins are always imprinted first, this sample per se imprinting method provides an effective and convenient way to deplete abundant proteins from complex samples such as serums, meanwhile concentrating and collecting scarce species therein. View Full-Text
Keywords: molecularly imprinted polymer; cryogel; bovine serum; abundant protein; depletion molecularly imprinted polymer; cryogel; bovine serum; abundant protein; depletion

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Yang, C.; Zhang, Y.; Cao, W.-Q.; Ji, X.-F.; Wang, J.; Yan, Y.-N.; Zhong, T.-L.; Wang, Y. Synthesis of Molecularly Imprinted Cryogels to Deplete Abundant Proteins from Bovine Serum. Polymers 2018, 10, 97.

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