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Polymers
Volume 10
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10.3390/polym10010054
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Article

Facile and Rapid Formation of Giant Vesicles from Glass Beads

by
Radu Tanasescu
1,
Ute Mettal
1,
Adai Colom
2,3,
Aurélien Roux
2,3 and
Andreas Zumbuehl
1,3,*
1
Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland
2
Department of Biochemistry, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva, Switzerland
3
National Centre of Competence in Research in Chemical Biology, 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
Polymers 2018, 10(1), 54; https://doi.org/10.3390/polym10010054
Submission received: 8 December 2017 / Revised: 31 December 2017 / Accepted: 4 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Selected Papers from "ECIS 2017")
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Abstract

Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
Keywords: formulation techniques; glass bead technique; giant vesicles; hybrid vesicles; phospholipids formulation techniques; glass bead technique; giant vesicles; hybrid vesicles; phospholipids
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MDPI and ACS Style

Tanasescu, R.; Mettal, U.; Colom, A.; Roux, A.; Zumbuehl, A. Facile and Rapid Formation of Giant Vesicles from Glass Beads. Polymers 2018, 10, 54. https://doi.org/10.3390/polym10010054

AMA Style

Tanasescu R, Mettal U, Colom A, Roux A, Zumbuehl A. Facile and Rapid Formation of Giant Vesicles from Glass Beads. Polymers. 2018; 10(1):54. https://doi.org/10.3390/polym10010054

Chicago/Turabian Style

Tanasescu, Radu, Ute Mettal, Adai Colom, Aurélien Roux, and Andreas Zumbuehl. 2018. "Facile and Rapid Formation of Giant Vesicles from Glass Beads" Polymers 10, no. 1: 54. https://doi.org/10.3390/polym10010054

APA Style

Tanasescu, R., Mettal, U., Colom, A., Roux, A., & Zumbuehl, A. (2018). Facile and Rapid Formation of Giant Vesicles from Glass Beads. Polymers, 10(1), 54. https://doi.org/10.3390/polym10010054

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