Comparison of Candida Albicans Fatty Acid Amide Hydrolase Structure with Homologous Amidase Signature Family Enzymes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cloning of FAAH from Candida Albicans
2.2. Purification of Recombinant Proteins
2.3. Crystallization
2.4. Data Collection and Structure Determination
2.5. Phylogenetic Analysis
3. Results
3.1. Overall Structure of CaFAAH
3.2. Active Site
3.3. Comparison of CaFAAH Structure with the AS Family Protein RnFAAH
3.4. Comparison of CaFAAH Structure with AS Family Proteins BjMAE2 and SmFAM
3.5. Comparison of CaFAAH Active Site with the RnFAAH-Inhibitor Complexes
3.6. Phylogenetic Analysis of AS Family Proteins
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Crystallographic Data | CaFAAH |
---|---|
Data collection | |
Space group | P21 |
Cell dimensions | |
a, b, c (Å) | 84.8, 68.7, 100.9 |
α, β, γ (˚) | 90, 99.6, 90 |
Resolution range (Å) | 50.0–2.28 (2.28–2.20) a |
Rmerge (%) b | 10.4 (64.6) |
I / sigmaI | 13.03 (4.66) |
Completeness (%) | 99.8 (98.5) |
Redundancy | 7.0 (7.4) |
Refinement | |
Resolution | 50.0–2.28 |
No. of reflections | 58833 |
Rworkc / Rfree (%) d | 17.27 / 21.97 |
No. of atoms | 9560 |
Protein | 8962 |
Water | 598 |
B-factors | |
Protein | 33.40 |
R.M.S. deviation | |
Bond lengths (Å) | 0.009 |
Bond angles (˚) | 1.21 |
PDB code | 6KVR |
Protein | Species | NCBI Reference Sequence |
---|---|---|
FAAH (Fatty acid amide hydrolase) | Danio rerio | NP_001103295.1 |
Homo sapiens | NP_001432.2 | |
Xenopus laevis | OCT82705.1 | |
Bos Taurus | DAA31035.1 | |
Rattus norvegicus | NP_077046.1 | |
Glutamyl-tRNA (Gln) Amidotransferase | Helicobacter pylori | WP_000631451.1 |
Listeria monocytogenes | WP_061104924.1 | |
Malonamidase | Bradyrhizobium Japonicum | WP_011087863.1 |
Peptide Amidase | Stenotrophomonas maltophilia | CAC93616.1 |
Amidase | Rhizobiales bacterium | WP_037017835.1 |
Pseudomonas geniculate | WP_057502485.1 | |
Haloterrigena jeotgali | WP_049965501.1 | |
Methylobacterium radiotolerans | WP_012319231.1 | |
Saccharomyces cerevisiae * | CAA39514.1 | |
Penicillium italicum | KGO69811 | |
Debaryomyces hansenii * | XP_457833.2 | |
Kluyveromyces lactis * | XP_451920.1 | |
Candida albicans * | XP_716991.1 | |
Schizosaccharomyces pombe * | NP_588099.1 |
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Share and Cite
Min, C.-A.; Yun, J.-S.; Choi, E.H.; Hwang, U.W.; Cho, D.-H.; Yoon, J.-H.; Chang, J.H. Comparison of Candida Albicans Fatty Acid Amide Hydrolase Structure with Homologous Amidase Signature Family Enzymes. Crystals 2019, 9, 472. https://doi.org/10.3390/cryst9090472
Min C-A, Yun J-S, Choi EH, Hwang UW, Cho D-H, Yoon J-H, Chang JH. Comparison of Candida Albicans Fatty Acid Amide Hydrolase Structure with Homologous Amidase Signature Family Enzymes. Crystals. 2019; 9(9):472. https://doi.org/10.3390/cryst9090472
Chicago/Turabian StyleMin, Cho-Ah, Ji-Sook Yun, Eun Hwa Choi, Ui Wook Hwang, Dong-Hyung Cho, Je-Hyun Yoon, and Jeong Ho Chang. 2019. "Comparison of Candida Albicans Fatty Acid Amide Hydrolase Structure with Homologous Amidase Signature Family Enzymes" Crystals 9, no. 9: 472. https://doi.org/10.3390/cryst9090472