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Correction: Taberman, H. Radiation Damage in Macromolecular Crystallography—An Experimentalist’s View. Crystals 2018, 8, 157
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Correction published on 20 October 2018, see Crystals 2018, 8(10), 393.

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Radiation Damage in Macromolecular Crystallography—An Experimentalist’s View

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Crystals 2018, 8(4), 157; https://doi.org/10.3390/cryst8040157
Received: 5 March 2018 / Revised: 22 March 2018 / Accepted: 24 March 2018 / Published: 4 April 2018
(This article belongs to the Special Issue Recent Advances in Protein Crystallography)
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Abstract

Radiation damage still remains a major limitation and challenge in macromolecular X-ray crystallography. Some of the high-intensity radiation used for diffraction data collection experiments is absorbed by the crystals, generating free radicals. These give rise to radiation damage even at cryotemperatures (~100 K), which can lead to incorrect biological conclusions being drawn from the resulting structure, or even prevent structure solution entirely. Investigation of mitigation strategies and the effects caused by radiation damage has been extensive over the past fifteen years. Here, recent understanding of the physical and chemical phenomena of radiation damage is described, along with the global effects inflicted on the collected data and the specific effects observed in the solved structure. Furthermore, this review aims to summarise the progress made in radiation damage studies in macromolecular crystallography from the experimentalist’s point of view and to give an introduction to the current literature. View Full-Text
Keywords: X-ray radiation damage; macromolecular crystallography; dose; global damage; site specific damage; radicals X-ray radiation damage; macromolecular crystallography; dose; global damage; site specific damage; radicals
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Taberman, H. Radiation Damage in Macromolecular Crystallography—An Experimentalist’s View. Crystals 2018, 8, 157.

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