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Article

Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates

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Laboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
2
Laboratory for Magnetic Resonances, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
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Department of Histology and Embryology, School of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, Croatia
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Center for Marine Research, Ruđer Bošković Institute, Giordano Paliaga 5, 52210 Rovinj, Croatia
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Institute for Physics, Bijenička cesta 46, 10000 Zagreb, Croatia
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Laboratory for Precipitation Processes, Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editors: Martina Medvidović-Kosanović, Jasminka Kontrec, Branka Njegić Džakula and Anamarija Stanković
Crystals 2021, 11(7), 792; https://doi.org/10.3390/cryst11070792
Received: 14 June 2021 / Revised: 1 July 2021 / Accepted: 4 July 2021 / Published: 7 July 2021
(This article belongs to the Special Issue Green Approach in Synthesis of Bio-Inspired Materials)
Understanding the effect that specific amino acids (AA) exert on calcium phosphate (CaPs) formation is proposed as a way of providing deeper insight into CaPs’ biomineralization and enabling the design of tailored-made additives for the synthesis of functional materials. Despite a number of investigations, the role of specific AA is still unclear, mostly because markedly different experimental conditions have been employed in different studies. The aim of this paper was to compare the influence of different classes of amino acids, charged (aspartic acid, Asp and lysine, Lys), polar (asparagine, Asn and serine, Ser) and non-polar (phenylalanine, Phe) on CaPs formation and transformation in conditions similar to physiological conditions. The precipitation process was followed potentiometrically, while Fourier transform infrared spectroscopy, powder X-ray diffraction, electron paramagnetic spectroscopy (EPR), scanning and transmission electron microscopy were used for the characterization of precipitates. Except for Phe, all investigated AAs inhibited amorphous calcium phosphate (ACP) transformation, with Ser being the most efficient inhibitor. In all systems, ACP transformed in calcium-deficient hydroxyapatite (CaDHA). However, the size of crystalline domains was affected, as well as CaDHA morphology. In EPR spectra, the contribution of different radical species with different proportions in diverse surroundings, depending on the type of AA present, was observed. The obtained results are of interest for the preparation of functionalized CaPs’, as well as for the understanding of their formation in vivo. View Full-Text
Keywords: amino acids; calcium phosphates; spontaneous precipitation; transformation amino acids; calcium phosphates; spontaneous precipitation; transformation
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MDPI and ACS Style

Erceg, I.; Maltar-Strmečki, N.; Jurašin, D.D.; Strasser, V.; Ćurlin, M.; Lyons, D.M.; Radatović, B.; Mlinarić, N.M.; Kralj, D.; Sikirić, M.D. Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates. Crystals 2021, 11, 792. https://doi.org/10.3390/cryst11070792

AMA Style

Erceg I, Maltar-Strmečki N, Jurašin DD, Strasser V, Ćurlin M, Lyons DM, Radatović B, Mlinarić NM, Kralj D, Sikirić MD. Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates. Crystals. 2021; 11(7):792. https://doi.org/10.3390/cryst11070792

Chicago/Turabian Style

Erceg, Ina, Nadica Maltar-Strmečki, Darija Domazet Jurašin, Vida Strasser, Marija Ćurlin, Daniel Mark Lyons, Borna Radatović, Nives Matijaković Mlinarić, Damir Kralj, and Maja Dutour Sikirić. 2021. "Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates" Crystals 11, no. 7: 792. https://doi.org/10.3390/cryst11070792

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