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Towards an Optimal Sample Delivery Method for Serial Crystallography at XFEL
Communication

Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff

Sample, Environment, and Delivery Group, Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
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Crystals 2020, 10(4), 251; https://doi.org/10.3390/cryst10040251
Received: 5 March 2020 / Revised: 24 March 2020 / Accepted: 24 March 2020 / Published: 27 March 2020
(This article belongs to the Special Issue Macromolecular Serial Crystallography)
Serial Femtosecond Crystallography (SFX) at X-ray Free electron Lasers (XFELs) is a relatively new field promising to deliver unparalleled spatial and temporal resolution on biological systems and there dynamics. Over the past decade, though, there have been a handful of results that have truly delivered on these promises. Why? SFX has many paradigm shifting techniques not seen in typical structural biology arenas, such as creating a concentrated slurry of microcrystals rather than a handful of loopable prisms worthy of a catalog photo. Then taking that slurry and high speed jetting them towards the vacuum or helium interation region to destroy less than 1% of your sample and waste the other 99. The literature is full of techniques and methods promising to cure what ails your experiment, yet as an instrument scientist will tell you –and a first author might admit after a few drinks at the conference happy hour—is that there are a lot more failures than the success we published, results may vary. We will walk through a best practices on how to prepare your sample and chose a sample delivery technique that will amerliorate your studies rather than undermine your hardwork and hopefully lead to better experimental planning and execution, inching you closer to that scientific goal and that call from Stockholm. This will be written in a more editorialized fashion and is meant to give the reader an idea of what to try or how they should be thinking. Welcome to SFX, now what? View Full-Text
Keywords: Serial Femtosecond Crystallography; X-ray Free Electron Laser; Sample Delivery; Sample Preparation; New Users Serial Femtosecond Crystallography; X-ray Free Electron Laser; Sample Delivery; Sample Preparation; New Users
MDPI and ACS Style

Kupitz, C.; Sierra, R.G. Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff. Crystals 2020, 10, 251. https://doi.org/10.3390/cryst10040251

AMA Style

Kupitz C, Sierra RG. Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff. Crystals. 2020; 10(4):251. https://doi.org/10.3390/cryst10040251

Chicago/Turabian Style

Kupitz, Christopher, and Raymond G. Sierra 2020. "Preventing Bio-Bloopers and XFEL Follies: Best Practices from your Friendly Instrument Staff" Crystals 10, no. 4: 251. https://doi.org/10.3390/cryst10040251

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