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Understanding (R) Specific Carbonyl Reductase from Candida parapsilosis ATCC 7330 [CpCR]: Substrate Scope, Kinetic Studies and the Role of Zinc

1
Laboratory of Bioorganic Chemistry, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India
2
National Centre for Catalysis Research, Indian Institute of Technology Madras, Chennai 600036, India
*
Author to whom correspondence should be addressed.
Catalysts 2019, 9(9), 702; https://doi.org/10.3390/catal9090702
Received: 26 July 2019 / Revised: 16 August 2019 / Accepted: 18 August 2019 / Published: 21 August 2019
(This article belongs to the Special Issue Biocatalysis: Chemical Biosynthesis)
CpCR, an (R) specific carbonyl reductase, so named because it gave (R)-alcohols on asymmetric reduction of ketones and ketoesters, is a recombinantly expressed enzyme from Candida parapsilosis ATCC 7330. It turns out to be a better aldehyde reductase and catalyses cofactor (NADPH) specific reduction of aliphatic and aromatic aldehydes. Kinetics studies against benzaldehyde and 2,4-dichlorobenzaldehyde show that the enzyme affinity and rate of reaction change significantly upon substitution on the benzene ring of benzaldehyde. CpCR, an MDR (medium chain reductase/dehydrogenase) containing both structural and catalytic Zn atoms, exists as a dimer, unlike the (S) specific reductase (SRED) from the same yeast which can exist in both dimeric and tetrameric forms. Divalent metal salts inhibit the enzyme even at nanomolar concentrations. EDTA chelation decreases CpCR activity. However, chelation done after the enzyme is pre-incubated with the NADPH retains most of the activity implying that Zn removal is largely prevented by the formation of the enzyme-cofactor complex. View Full-Text
Keywords: MDR—medium-chain reductase/dehydrogenase; ADH—alcohol dehydrogenase; enzyme kinetics; EDTA (Ethylenediaminetetraacetic acid) chelation; ultrafiltration MDR—medium-chain reductase/dehydrogenase; ADH—alcohol dehydrogenase; enzyme kinetics; EDTA (Ethylenediaminetetraacetic acid) chelation; ultrafiltration
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Karanam, V.K.; Chaudhury, D.; Chadha, A. Understanding (R) Specific Carbonyl Reductase from Candida parapsilosis ATCC 7330 [CpCR]: Substrate Scope, Kinetic Studies and the Role of Zinc. Catalysts 2019, 9, 702.

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