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Open AccessArticle

Catalytic Activities of Multimeric G-Quadruplex DNAzymes

1
Department of Biochemistry, University of Ilorin, Ilorin 240003, Kwara State, Nigeria
2
School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, UK
*
Author to whom correspondence should be addressed.
Catalysts 2019, 9(7), 613; https://doi.org/10.3390/catal9070613
Received: 9 July 2019 / Accepted: 15 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Biocatalytic Applications in Biotechnology)
G-quadruplex DNAzymes are short DNA aptamers with repeating G4 quartets bound in a non-covalent complex with hemin. These G4/Hemin structures exhibit versatile peroxidase-like catalytic activity with a wide range of potential applications in biosensing and biotechnology. Current efforts are aimed at gaining a better understanding of the molecular mechanism of DNAzyme catalysis as well as devising strategies for improving their catalytic efficiency. Multimerisation of discrete units of G-quadruplexes to form multivalent DNAzyes is an emerging design strategy aimed at enhancing the peroxidase activities of DNAzymes. While this approach holds promise of generating more active multivalent G-quadruplex DNAzymes, few examples have been studied and it is not clear what factors determine the enhancement of catalytic activities of multimeric DNAzymes. In this study, we report the design and characterisation of multimers of five G-quadruplex sequences (AS1411, Bcl-2, c-MYC, PS5.M and PS2.M). Our results show that multimerisation of G-quadruplexes that form parallel structure (AS1411, Bcl-2, c-MYC) leads to significant rate enhancements characteristic of cooperative and/or synergistic interactions between the monomeric units. In contrast, multimerisation of DNA sequences that form non-parallel structures (PS5.M and PS2.M) did not exhibit similar levels of synergistic increase in activities. These results show that design of multivalent G4/Hemin structures could lead to a new set of versatile and efficient DNAzymes with enhanced capacity to catalyse peroxidase-mimic reactions. View Full-Text
Keywords: G-quadruplex; DNAzymes; peroxidase; multimers; synergism G-quadruplex; DNAzymes; peroxidase; multimers; synergism
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MDPI and ACS Style

Adeoye, R.I.; Osalaye, D.S.; Ralebitso-Senior, T.K.; Boddis, A.; Reid, A.J.; Fatokun, A.A.; Powell, A.K.; Malomo, S.O.; Olorunniji, F.J. Catalytic Activities of Multimeric G-Quadruplex DNAzymes. Catalysts 2019, 9, 613. https://doi.org/10.3390/catal9070613

AMA Style

Adeoye RI, Osalaye DS, Ralebitso-Senior TK, Boddis A, Reid AJ, Fatokun AA, Powell AK, Malomo SO, Olorunniji FJ. Catalytic Activities of Multimeric G-Quadruplex DNAzymes. Catalysts. 2019; 9(7):613. https://doi.org/10.3390/catal9070613

Chicago/Turabian Style

Adeoye, Raphael I.; Osalaye, Dunsin S.; Ralebitso-Senior, Theresia K.; Boddis, Amanda; Reid, Amanda J.; Fatokun, Amos A.; Powell, Andrew K.; Malomo, Sylvia O.; Olorunniji, Femi J. 2019. "Catalytic Activities of Multimeric G-Quadruplex DNAzymes" Catalysts 9, no. 7: 613. https://doi.org/10.3390/catal9070613

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