Kinetic Study of Peroxidase-Catalyzed Oxidation of 2-Hydroxyanthracene and 9-Phenanthrol in Presence of Biosurfactant Escin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Kinetics of Peroxidase-Catalyzed 2-Hydroxyanthracene and 9-Phenanthrol Oxidation in the Absence and Presence of Biosurfactant Escin
2.2. Enhancing Effect of Escin Monomers on Biocatalytic Oxidation of 2-Hydroxyanthracene and 9-Phenanthrol
2.3. Mechanism of Escin Action
3. Materials and Methods
3.1. Materials
3.2. CMC Determination
3.3. Kinetic Analysis
3.4. Mathematical Calculations
4. Conclusions
Funding
Acknowledgments
Conflicts of Interest
References
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AHDs | Escin, μmol L−1 | k2, mol−1 L s−1 | k3, mol−1 L s−1 | kin, mol−1 L s−1 |
---|---|---|---|---|
2-hydroxyanthracene | 0 | (1.0 ± 0.3) × 108 | (1.0 ± 0.2) × 108 | (2.2 ± 0.1) × 104 |
2-hydroxyanthracene | 0.2 | (1.0 ± 0.2) × 108 | (1.0 ± 0.3) × 108 | (9.5 ± 0.3) × 103 |
2-hydroxyanthracene | 0.5 | (1.0 ± 0.3) × 108 | (1.0 ± 0.2) × 108 | (5.3 ± 0.2) × 103 |
2-hydroxyanthracene | 0.7 | (9.2 ± 0.5) × 107 | (9.0 ± 0.5) × 107 | (4.2 ± 0.2) × 103 |
2-hydroxyanthracene | 1 | (9.0 ± 0.6) × 107 | (9.5 ± 0.4) × 107 | (1.6 ± 0.3) × 103 |
2-hydroxyanthracene | 2 μmol L−1–0.2 mmol L−1 | (1.0 ± 0.2) × 108 | (1.0 ± 0.2) × 108 | (9.0 ± 0.2) × 102 |
9-phenanthrol | 0 | (3.0 ± 0.1) × 107 | (3.0 ± 0.1) × 107 | (5.0 ± 0.2) × 104 |
9-phenanthrol | 0.2 | (3.0 ± 0.1) × 107 | (3.1 ± 0.1) × 107 | (3.3 ± 0.4) × 104 |
9-phenanthrol | 0.5 | (3.0 ± 0.1) × 107 | (3.0 ± 0.1) × 107 | (1.5 ± 0.3) × 104 |
9-phenanthrol | 0.7 | (3.2 ± 0.2) × 107 | (3.3 ± 0.2) × 107 | (7.4 ± 0.2) × 103 |
9-phenanthrol | 1 | (3.0 ± 0.1) × 107 | (3.0 ± 0.2) × 107 | (3.3 ± 0.1) × 103 |
9-phenanthrol | 2 μmol L−1–0.2 mmol L−1 | (3.1 ± 0.2) × 107 | (3.0 ± 0.1) × 107 | (1.5 ± 0.1) × 103 |
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Ivanec-Goranina, R. Kinetic Study of Peroxidase-Catalyzed Oxidation of 2-Hydroxyanthracene and 9-Phenanthrol in Presence of Biosurfactant Escin. Catalysts 2019, 9, 591. https://doi.org/10.3390/catal9070591
Ivanec-Goranina R. Kinetic Study of Peroxidase-Catalyzed Oxidation of 2-Hydroxyanthracene and 9-Phenanthrol in Presence of Biosurfactant Escin. Catalysts. 2019; 9(7):591. https://doi.org/10.3390/catal9070591
Chicago/Turabian StyleIvanec-Goranina, Rūta. 2019. "Kinetic Study of Peroxidase-Catalyzed Oxidation of 2-Hydroxyanthracene and 9-Phenanthrol in Presence of Biosurfactant Escin" Catalysts 9, no. 7: 591. https://doi.org/10.3390/catal9070591