Next Article in Journal
The Effect of Citric Acid Concentration on the Properties of LaMnO3 as a Catalyst for Hydrocarbon Oxidation
Next Article in Special Issue
Characterisation of the First Archaeal Mannonate Dehydratase from Thermoplasma acidophilum and Its Potential Role in the Catabolism of D-Mannose
Previous Article in Journal
Cross-Linking with Polyethylenimine Confers Better Functional Characteristics to an Immobilized β-glucosidase from Exiguobacterium antarcticum B7
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Catalysts 2019, 9(3), 225; https://doi.org/10.3390/catal9030225

An Innovative Biocatalyst for Continuous 2G Ethanol Production from Xylo-Oligomers by Saccharomyces cerevisiae through Simultaneous Hydrolysis, Isomerization, and Fermentation (SHIF)

1
Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil
2
Graduate Program of Chemical Engineering, Federal University of São Carlos (PPGEQ-UFSCar), Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work
Received: 3 February 2019 / Revised: 21 February 2019 / Accepted: 24 February 2019 / Published: 1 March 2019
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysts)
Full-Text   |   PDF [1226 KB, uploaded 1 March 2019]   |  

Abstract

Many approaches have been considered aimed at ethanol production from the hemicellulosic fraction of biomass. However, the industrial implementation of this process has been hindered by some bottlenecks, one of the most important being the ease of contamination of the bioreactor by bacteria that metabolize xylose. This work focuses on overcoming this problem through the fermentation of xylulose (the xylose isomer) by native Saccharomyces cerevisiae using xylo-oligomers as substrate. A new concept of biocatalyst is proposed, containing xylanases and xylose isomerase (XI) covalently immobilized on chitosan, and co-encapsulated with industrial baker’s yeast in Ca-alginate gel spherical particles. Xylo-oligomers are hydrolyzed, xylose is isomerized, and finally xylulose is fermented to ethanol, all taking place simultaneously, in a process called simultaneous hydrolysis, isomerization, and fermentation (SHIF). Among several tested xylanases, Multifect CX XL A03139 was selected to compose the biocatalyst bead. Influences of pH, Ca2+, and Mg2+ concentrations on the isomerization step were assessed. Experiments of SHIF using birchwood xylan resulted in an ethanol yield of 0.39 g/g, (76% of the theoretical), selectivity of 3.12 gethanol/gxylitol, and ethanol productivity of 0.26 g/L/h. View Full-Text
Keywords: 2G ethanol; hemicellulose usage; S. cerevisiae; enzyme immobilization; cell immobilization; SHIF 2G ethanol; hemicellulose usage; S. cerevisiae; enzyme immobilization; cell immobilization; SHIF
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Milessi-Esteves, T.S.; Corradini, F.A.; Kopp, W.; Zangirolami, T.C.; Tardioli, P.W.; Giordano, R.C.; Giordano, R.L. An Innovative Biocatalyst for Continuous 2G Ethanol Production from Xylo-Oligomers by Saccharomyces cerevisiae through Simultaneous Hydrolysis, Isomerization, and Fermentation (SHIF). Catalysts 2019, 9, 225.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Catalysts EISSN 2073-4344 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top