Enhancing Enzymatic Properties of Endoglucanase I Enzyme from Trichoderma Reesei via Swapping from Cellobiohydrolase I Enzyme
Abstract
:1. Introduction
2. Results & Discussion
2.1. Construction of EGI_Swapped and Cloning Approach
2.2. Enzymatic Activities
2.3. Thermal Stabilities of EGI and EGI_Swapped
2.4. Analysis of Coordinated Co2+ Ions in the Structures of EGI and EGI_Swapped Enzymes
2.5. The Change in Secondary Structures Upon Swapping
3. Material & Methods
3.1. The Construction of EGI_Swapped and Cloning Approach
3.2. P. pastoris Expression & Purification
3.3. Gel Electrophoresis
3.4. Bioinformatic Analysis of EGI and EGI_Swapped
3.5. Enzyme Assays
3.6. Thermal Stability Assay
3.7. Measurement of Co(II) Ion Concentration in EGI and EGI_Swapped Enzymes
3.8. Circular Dichroism (CD) Spectroscopy Measurements
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
Cellobiohydrolase I | CBHI |
CD | Circular Dichroism |
Carboxymethyl cellulase | CMC |
3,5-Dinitrosalicylic acid | DNS |
Endoglucanese I | EGI |
Endoglucanese I swapped | EGI_swapped |
PDB | Protein Data Bank |
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Yenenler, A.; Kurt, H.; Sezerman, O.U. Enhancing Enzymatic Properties of Endoglucanase I Enzyme from Trichoderma Reesei via Swapping from Cellobiohydrolase I Enzyme. Catalysts 2019, 9, 130. https://doi.org/10.3390/catal9020130
Yenenler A, Kurt H, Sezerman OU. Enhancing Enzymatic Properties of Endoglucanase I Enzyme from Trichoderma Reesei via Swapping from Cellobiohydrolase I Enzyme. Catalysts. 2019; 9(2):130. https://doi.org/10.3390/catal9020130
Chicago/Turabian StyleYenenler, Aslı, Hasan Kurt, and Osman Uğur Sezerman. 2019. "Enhancing Enzymatic Properties of Endoglucanase I Enzyme from Trichoderma Reesei via Swapping from Cellobiohydrolase I Enzyme" Catalysts 9, no. 2: 130. https://doi.org/10.3390/catal9020130