Next Article in Journal
A Polypyrrole-Modified Pd-Ag Bimetallic Electrode for the Electrocatalytic Reduction of 4-Chlorophenol
Previous Article in Journal
Deactivation of Cu/SSZ-13 NH3-SCR Catalyst by Exposure to CO, H2, and C3H6
Previous Article in Special Issue
Deciphering the Effect of Microbead Size Distribution on the Kinetics of Heterogeneous Biocatalysts through Single-Particle Analysis Based on Fluorescence Microscopy
Open AccessArticle

Estimation of the Effectiveness Factor for Immobilized Enzyme Catalysts through a Simple Conversion Assay

Department of Chemical and Environmental Engineering, Technical University Federico Santa María, PO Box 110-V, Valparaíso 2340000, Chile
Author to whom correspondence should be addressed.
Catalysts 2019, 9(11), 930;
Received: 30 September 2019 / Revised: 22 October 2019 / Accepted: 25 October 2019 / Published: 7 November 2019
A novel methodology to estimate the effectiveness factor (EF) of an immobilized enzyme catalyst is proposed here. The methodology consists of the determination of the productivity of both the immobilized enzyme catalyst and its corresponding soluble enzyme, plotted as a function of the reaction conversion. The ratio of these productivities corresponds to the EF estimator of the catalyst. Conversion curves were simulated in a batch reactor with immobilized enzyme and soluble enzyme for different values of the S0/KM ratio and Thiele modulus (Φ) to demonstrate this hypothesis. Two different reaction orders were tested: first-order kinetic and Michaelis–Menten-based kinetic with product inhibition. The results showed that the ratio of productivities between the immobilized and soluble enzymes followed the behavior profile presented by the EF with satisfactory agreement. This simple methodology to estimate the EF is based on routine conversion experiments, thus avoiding the exhaustive kinetic and mass transfer characterization of the immobilized enzyme catalyst. View Full-Text
Keywords: effectiveness factor; immobilized enzyme; diffusional restrictions effectiveness factor; immobilized enzyme; diffusional restrictions
Show Figures

Graphical abstract

MDPI and ACS Style

Valencia, P.; Ibañez, F. Estimation of the Effectiveness Factor for Immobilized Enzyme Catalysts through a Simple Conversion Assay. Catalysts 2019, 9, 930.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop