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Open AccessArticle

Estimation of the Effectiveness Factor for Immobilized Enzyme Catalysts through a Simple Conversion Assay

Department of Chemical and Environmental Engineering, Technical University Federico Santa María, PO Box 110-V, Valparaíso 2340000, Chile
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Catalysts 2019, 9(11), 930; https://doi.org/10.3390/catal9110930
Received: 30 September 2019 / Revised: 22 October 2019 / Accepted: 25 October 2019 / Published: 7 November 2019
A novel methodology to estimate the effectiveness factor (EF) of an immobilized enzyme catalyst is proposed here. The methodology consists of the determination of the productivity of both the immobilized enzyme catalyst and its corresponding soluble enzyme, plotted as a function of the reaction conversion. The ratio of these productivities corresponds to the EF estimator of the catalyst. Conversion curves were simulated in a batch reactor with immobilized enzyme and soluble enzyme for different values of the S0/KM ratio and Thiele modulus (Φ) to demonstrate this hypothesis. Two different reaction orders were tested: first-order kinetic and Michaelis–Menten-based kinetic with product inhibition. The results showed that the ratio of productivities between the immobilized and soluble enzymes followed the behavior profile presented by the EF with satisfactory agreement. This simple methodology to estimate the EF is based on routine conversion experiments, thus avoiding the exhaustive kinetic and mass transfer characterization of the immobilized enzyme catalyst. View Full-Text
Keywords: effectiveness factor; immobilized enzyme; diffusional restrictions effectiveness factor; immobilized enzyme; diffusional restrictions
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MDPI and ACS Style

Valencia, P.; Ibañez, F. Estimation of the Effectiveness Factor for Immobilized Enzyme Catalysts through a Simple Conversion Assay. Catalysts 2019, 9, 930.

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