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Article

Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

1
School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Korea
2
Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Anseo-Dong, Cheonan 31116, Korea
3
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 6397798, Singapore
4
Department of Microbiology, College of Natural Science, Dankook University, Cheonan 31116, Korea
5
Department of Pathology, CHA University, Pocheon-si 11160, Korea
6
Department of Biomedical Science, College of Life Science, CHA University, Pocheon-si 11160, Korea
7
Department of Chemistry, College of Natural Science, Dankook University, Anseo-dong, Cheonan 31116, Korea
8
CHA Fertility Center Seoul Station, Jung-gu, Seoul 04637, Korea
*
Authors to whom correspondence should be addressed.
W.-Y. Jeon and S. Mun contributed equally to this paper.
Catalysts 2021, 11(1), 62; https://doi.org/10.3390/catal11010062
Received: 27 November 2020 / Revised: 18 December 2020 / Accepted: 31 December 2020 / Published: 5 January 2021
(This article belongs to the Special Issue Recent Advances in Enzyme Technology)
Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices. View Full-Text
Keywords: human adipose-derived mesenchymal stem cell; enzymatic biofuel cell; electrical stimulation; next generation sequencing; wearable bioelectronic devices human adipose-derived mesenchymal stem cell; enzymatic biofuel cell; electrical stimulation; next generation sequencing; wearable bioelectronic devices
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MDPI and ACS Style

Jeon, W.-Y.; Mun, S.; Ng, W.B.; Kang, K.; Han, K.; Hwang, S.; Kim, H.-H.; Lee, J.H. Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell. Catalysts 2021, 11, 62. https://doi.org/10.3390/catal11010062

AMA Style

Jeon W-Y, Mun S, Ng WB, Kang K, Han K, Hwang S, Kim H-H, Lee JH. Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell. Catalysts. 2021; 11(1):62. https://doi.org/10.3390/catal11010062

Chicago/Turabian Style

Jeon, Won-Yong, Seyoung Mun, Wei B. Ng, Keunsoo Kang, Kyudong Han, Sohyun Hwang, Hyug-Han Kim, and Jae H. Lee 2021. "Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell" Catalysts 11, no. 1: 62. https://doi.org/10.3390/catal11010062

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