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Review

Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

1
Eukaryotic Molecular Cell Biology, Synthetic Biology Section, Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, 2800 Kongens Lyngby, Denmark
2
Laboratory of Multidisciplinary Research, São Francisco University, USF, São Francisco de Assis Avenue, 218, 12916900 Bragança Paulista, SP, Brazil
3
Department of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, Viikinkaari 9, 00790 Helsinki, Finland
4
Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials(CNPEM), Campinas, 13083100 São Paulo, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Catalysts 2020, 10(9), 1032; https://doi.org/10.3390/catal10091032
Received: 27 July 2020 / Revised: 28 August 2020 / Accepted: 3 September 2020 / Published: 9 September 2020
(This article belongs to the Special Issue Industrial Biocatalysis: Challenges and Opportunities)
Lipases are one of the most used enzymes in the pharmaceutical industry due to their efficiency in organic syntheses, mainly in the production of enantiopure drugs. From an industrial viewpoint, the selection of an efficient expression system and host for recombinant lipase production is highly important. The most used hosts are Escherichia coli and Komagataella phaffii (previously known as Pichia pastoris) and less often reported Bacillus and Aspergillus strains. The use of efficient expression systems to overproduce homologous or heterologous lipases often require the use of strong promoters and the co-expression of chaperones. Protein engineering techniques, including rational design and directed evolution, are the most reported strategies for improving lipase characteristics. Additionally, lipases can be immobilized in different supports that enable improved properties and enzyme reuse. Here, we review approaches for strain and protein engineering, immobilization and the application of lipases in the pharmaceutical industry. View Full-Text
Keywords: biocatalysis; industrial applications; sustainable chemistry biocatalysis; industrial applications; sustainable chemistry
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MDPI and ACS Style

Contesini, F.J.; Davanço, M.G.; Borin, G.P.; Vanegas, K.G.; Cirino, J.P.G.; Melo, R.R.d.; Mortensen, U.H.; Hildén, K.; Campos, D.R.; Carvalho, P.d.O. Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry. Catalysts 2020, 10, 1032. https://doi.org/10.3390/catal10091032

AMA Style

Contesini FJ, Davanço MG, Borin GP, Vanegas KG, Cirino JPG, Melo RRd, Mortensen UH, Hildén K, Campos DR, Carvalho PdO. Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry. Catalysts. 2020; 10(9):1032. https://doi.org/10.3390/catal10091032

Chicago/Turabian Style

Contesini, Fabiano J., Marcelo G. Davanço, Gustavo P. Borin, Katherina G. Vanegas, João P.G. Cirino, Ricardo R.d. Melo, Uffe H. Mortensen, Kristiina Hildén, Daniel R. Campos, and Patricia d.O. Carvalho. 2020. "Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry" Catalysts 10, no. 9: 1032. https://doi.org/10.3390/catal10091032

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