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Open AccessArticle

Whole-Cells of Yarrowia lipolytica Applied in “One Pot” Indolizine Biosynthesis

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Department of Chemistry, Physics and Environment, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
2
Department of Food Science, Food Engineering and Applied Biotechnology, “Dunărea de Jos” University of Galati, 111 Domnească Street, 800201 Galati, Romania
3
Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu CS 50837, CEDEX 7, 35708 Rennes, France
*
Authors to whom correspondence should be addressed.
Catalysts 2020, 10(6), 629; https://doi.org/10.3390/catal10060629
Received: 9 May 2020 / Revised: 2 June 2020 / Accepted: 3 June 2020 / Published: 5 June 2020
(This article belongs to the Special Issue Biocatalytic Process Optimization)
A series of yeast strains was tested in order to evaluate their catalytic potential in biocatalysis of one-pot indolizine’s synthesis. Yeast cultivation was performed in a submerged system at 28 °C for 72 h at 180 rpm. An assessment of the reagents’ toxicity on yeast viability and metabolic functionality concluded that the growth potential of three Yarrowia lipolytica strains were least affected by the reactants compared to the other yeast strains. Further, crude fermentation products (biomass and cell-free supernatant)—obtained by submerged cultivation of these yeasts—were used in multistep cascade reactions for the production of fluorescent indolizine compounds with important biologic activities. A whole–cell catalyzed multicomponent reaction of activated alkynes, α-bromo-carbonyl reagents and 4,4′-bipyridine, at room temperature in buffer solution led to the efficient synthesis of bis-indolizines 4a, 4b and 4c, in good-to-excellent yields (47%–77%). The metabolites of the selected Y. lipolytica strains can be considered effective biocatalysts in cycloaddition reactions and the high purity and bioconversion yields of the synthesized indolizines indicates a great potential of this type of “green” catalysts. Seeds of Triticum estivum L. were used to investigate the impact of the final products on the germination and seedling growth. The most sensitive physiological parameters suggest that indolizines, at the concentrations tested, have non-toxic effect on germination and seedling growth of wheat, fact also confirmed by confocal laser scanning microscopy images. View Full-Text
Keywords: Yarrowia lipolytica; whole–cell biocatalysis; indolizine; cycloaddition Yarrowia lipolytica; whole–cell biocatalysis; indolizine; cycloaddition
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MDPI and ACS Style

Botezatu, A.V.; Horincar, G.; Ghinea, I.O.; Furdui, B.; Bahrim, G.-E.; Barbu, V.; Balanescu, F.; Favier, L.; Dinica, R.-M. Whole-Cells of Yarrowia lipolytica Applied in “One Pot” Indolizine Biosynthesis. Catalysts 2020, 10, 629.

AMA Style

Botezatu AV, Horincar G, Ghinea IO, Furdui B, Bahrim G-E, Barbu V, Balanescu F, Favier L, Dinica R-M. Whole-Cells of Yarrowia lipolytica Applied in “One Pot” Indolizine Biosynthesis. Catalysts. 2020; 10(6):629.

Chicago/Turabian Style

Botezatu, Andreea V.; Horincar, Georgiana; Ghinea, Ioana O.; Furdui, Bianca; Bahrim, Gabriela-Elena; Barbu, Vasilica; Balanescu, Fanica; Favier, Lidia; Dinica, Rodica-Mihaela. 2020. "Whole-Cells of Yarrowia lipolytica Applied in “One Pot” Indolizine Biosynthesis" Catalysts 10, no. 6: 629.

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