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Open AccessFeature PaperArticle

Synthesis of DHA/EPA Ethyl Esters via Lipase-Catalyzed Acidolysis Using Novozym® 435: A Kinetic Study

1
Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan
2
Department of Chemical and Materials Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 807, Taiwan
3
Department of Mechatronics Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan
4
Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan
*
Authors to whom correspondence should be addressed.
Catalysts 2020, 10(5), 565; https://doi.org/10.3390/catal10050565
Received: 14 April 2020 / Revised: 6 May 2020 / Accepted: 12 May 2020 / Published: 19 May 2020
(This article belongs to the Special Issue Biocatalytic Process Optimization)
DHA/EPA ethyl ester is mainly used in the treatment of arteriosclerosis and hyperlipidemia. In this study, DHA+EPA ethyl ester was synthesized via lipase-catalyzed acidolysis of ethyl acetate (EA) with DHA+EPA concentrate in n-hexane using Novozym® 435. The DHA+EPA concentrate (in free fatty acid form), contained 54.4% DHA and 16.8% EPA, was used as raw material. A central composite design combined with response surface methodology (RSM) was used to evaluate the relationship between substrate concentrations and initial rate of DHA+EPA ethyl ester production. The results indicated that the reaction followed the ordered mechanism and as such, the ordered mechanism model was used to estimate the maximum reaction rate (Vmax) and kinetic constants. The ordered mechanism model was also combined with the batch reaction equation to simulate and predict the conversion of DHA+EPA ethyl ester in lipase-catalyzed acidolysis. The integral equation showed a good predictive relationship between the simulated and experimental results. 88–94% conversion yields were obtained from 100–400 mM DHA+EPA concentrate at a constant enzyme activity of 200 U, substrate ratio of 1:1 (DHA+EPA: EA), and reaction time of 300 min. View Full-Text
Keywords: lipase; acidolysis; docosahexaenoic acid ethyl ester; eicosapentaenoic acid ethyl ester; ethyl acetate; kinetics lipase; acidolysis; docosahexaenoic acid ethyl ester; eicosapentaenoic acid ethyl ester; ethyl acetate; kinetics
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MDPI and ACS Style

Kuo, C.-H.; Huang, C.-Y.; Lee, C.-L.; Kuo, W.-C.; Hsieh, S.-L.; Shieh, C.-J. Synthesis of DHA/EPA Ethyl Esters via Lipase-Catalyzed Acidolysis Using Novozym® 435: A Kinetic Study. Catalysts 2020, 10, 565.

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