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Open AccessArticle

Bacillus subtilis Lipase A—Lipase or Esterase?

1
Biokatalyse, Afdeling Biotechnologie, Technische Universiteit Delft, Van der Maasweg 9, 2629 HZ Delft, The Netherlands
2
Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy
*
Author to whom correspondence should be addressed.
Catalysts 2020, 10(3), 308; https://doi.org/10.3390/catal10030308
Received: 20 February 2020 / Revised: 4 March 2020 / Accepted: 5 March 2020 / Published: 7 March 2020
(This article belongs to the Special Issue Novel Enzyme and Whole-Cell Biocatalysts)
The question of how to distinguish between lipases and esterases is about as old as the definition of the subclassification is. Many different criteria have been proposed to this end, all indicative but not decisive. Here, the activity of lipases in dry organic solvents as a criterion is probed on a minimal α/β hydrolase fold enzyme, the Bacillus subtilis lipase A (BSLA), and compared to Candida antarctica lipase B (CALB), a proven lipase. Both hydrolases show activity in dry solvents and this proves BSLA to be a lipase. Overall, this demonstrates the value of this additional parameter to distinguish between lipases and esterases. Lipases tend to be active in dry organic solvents, while esterases are not active under these circumstances. View Full-Text
Keywords: hydrolase; lipase; esterase; Bacillus subtilis lipase A; transesterification; organic solvent; water activity hydrolase; lipase; esterase; Bacillus subtilis lipase A; transesterification; organic solvent; water activity
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MDPI and ACS Style

Bracco, P.; van Midden, N.; Arango, E.; Torrelo, G.; Ferrario, V.; Gardossi, L.; Hanefeld, U. Bacillus subtilis Lipase A—Lipase or Esterase? Catalysts 2020, 10, 308.

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