KIF2C Facilitates Tumor Growth and Metastasis in Pancreatic Ductal Adenocarcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Survival Curve and Correlation Analysis
2.2. Patients and Specimens
2.3. Immunohistochemical (IHC) Staining
2.4. Cell Culture
2.5. Plasmid Construction and Transfection
2.6. Cell Proliferation, Migration, and Invasion Assays
2.7. RNA Isolation and Quantitative RT-PCR
2.8. Western Blot
2.9. Antibodies
2.10. Animals Model
2.11. Embedding and Slicing
2.12. Cell Cycle Detection
2.13. H&E Staining
2.14. Statistical Analysis
2.15. Data Availability Statement
2.16. Ethics
3. Results
3.1. Upregulation of KIF2C in PDAC
3.2. Abnormal Expression of KIF2C in Clinical Specimens
3.3. Knockdown of KIF2C Inhibits PDAC Cell Proliferation, Migration, and Invasion In Vitro
3.4. Overexpression of KIF2C Promotes PDAC Cell Proliferation, Migration, and Invasion In Vitro
3.5. KIF2C Promotes PDAC Cell Proliferation, Migration, and Invasion In Vivo
3.6. The Potential Mechanism of KIF2C in PDAC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Clinicopathological Variables | n | KIF2C Expression | p Value | |
---|---|---|---|---|
Low/Moderate | High | |||
All cases | 70 | 42 | 28 | |
Age (years) | ||||
<60 | 32 | 18 | 14 | |
≥60 | 38 | 16 | 22 | 0.643 |
Gender | ||||
Male | 35 | 23 | 12 | |
Female | 35 | 16 | 20 | 0.111 |
TNM stage | ||||
IA | 27 | 17 | 10 | |
IB | 17 | 8 | 9 | |
IIA | 0 | 0 | 0 | |
B | 11 | 4 | 7 | |
III | 0 | 0 | 0 | |
IV | 14 | 4 | 10 | 0.028 (<0.05) |
Differentiated degree | ||||
Low/moderate | 46 | 17 | 29 | |
High | 24 | 17 | 7 | 0.022 (<0.05) |
Drinking history | ||||
Yes | 21 | 12 | 9 | |
No | 49 | 22 | 27 | 0.412 |
Obstructive jaundice | ||||
Yes | 39 | 17 | 22 | |
No | 31 | 17 | 14 | 0.420 |
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Huang, X.; Zhao, F.; Wu, Q.; Wang, Z.; Ren, H.; Zhang, Q.; Wang, Z.; Xu, J. KIF2C Facilitates Tumor Growth and Metastasis in Pancreatic Ductal Adenocarcinoma. Cancers 2023, 15, 1502. https://doi.org/10.3390/cancers15051502
Huang X, Zhao F, Wu Q, Wang Z, Ren H, Zhang Q, Wang Z, Xu J. KIF2C Facilitates Tumor Growth and Metastasis in Pancreatic Ductal Adenocarcinoma. Cancers. 2023; 15(5):1502. https://doi.org/10.3390/cancers15051502
Chicago/Turabian StyleHuang, Xing, Feng Zhao, Quan Wu, Zitong Wang, Haiyue Ren, Qiqi Zhang, Zhe Wang, and Jin Xu. 2023. "KIF2C Facilitates Tumor Growth and Metastasis in Pancreatic Ductal Adenocarcinoma" Cancers 15, no. 5: 1502. https://doi.org/10.3390/cancers15051502