An Optimized Single Nucleotide Polymorphism-Based Detection Method Suggests That Allelic Variants in the 3’ Untranslated Region of RRAS2 Correlate with Treatment Response in Chronic Lymphocytic Leukemia Patients
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Human Cells
2.2. Flow Cytometry
2.3. gDNA Extraction
2.4. Sequencing Strategy for Patients’ Samples
2.5. RRAS2 Expression Measurement
2.6. Fluorescent Probes Method to Sequence Patients’ Samples
3. Statistical Analysis
4. Results
4.1. A Novel qPCR Method Greatly Improves Sensitivity and Time Efficiency of rs8570 SNP Characterization
4.2. Treated CLL Patients with at Least One C Allele at rs8570 Show a Better Response to Ibrutinib
5. Discussion
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hortal, A.; Lacuna, M.; Cifuentes, C.; Alcoceba, M.; Bustelo, X.R.; González, M.; Alarcón, B. An Optimized Single Nucleotide Polymorphism-Based Detection Method Suggests That Allelic Variants in the 3’ Untranslated Region of RRAS2 Correlate with Treatment Response in Chronic Lymphocytic Leukemia Patients. Cancers 2023, 15, 644. https://doi.org/10.3390/cancers15030644
Hortal A, Lacuna M, Cifuentes C, Alcoceba M, Bustelo XR, González M, Alarcón B. An Optimized Single Nucleotide Polymorphism-Based Detection Method Suggests That Allelic Variants in the 3’ Untranslated Region of RRAS2 Correlate with Treatment Response in Chronic Lymphocytic Leukemia Patients. Cancers. 2023; 15(3):644. https://doi.org/10.3390/cancers15030644
Chicago/Turabian StyleHortal, Alejandro, Marta Lacuna, Claudia Cifuentes, Miguel Alcoceba, Xosé R. Bustelo, Marcos González, and Balbino Alarcón. 2023. "An Optimized Single Nucleotide Polymorphism-Based Detection Method Suggests That Allelic Variants in the 3’ Untranslated Region of RRAS2 Correlate with Treatment Response in Chronic Lymphocytic Leukemia Patients" Cancers 15, no. 3: 644. https://doi.org/10.3390/cancers15030644