LLGL2 Inhibits Ovarian Cancer Metastasis by Regulating Cytoskeleton Remodeling via ACTN1
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Bioinformatics Analysis
2.2. Cell Lines
2.3. Transfections and Lentiviral Infection
2.4. Western Blot Analysis
2.5. Transwell and Wound Healing Assays
2.6. Phalloidin Staining
2.7. Coimmunoprecipitation (Co-IP) and Liquid Chromatography–Mass Spectrometry
2.8. Immunofluorescence Staining
2.9. Tumor Xenograft Models
2.10. Immunohistochemistry (IHC)
2.11. Statistical Analysis
3. Results
3.1. Identification of LLGL2 as a Key Gene in Ovarian Cancer
3.2. LLGL2 Correlated with Tumor Progression and a Better Prognosis in Ovarian Cancer
3.3. LLGL2 Inhibited Ovarian Cancer Cell Migration and Invasion In Vitro
3.4. LLGL2 Suppressed the Dissemination of Ovarian Cancer Cells In Vivo
3.5. LLGL2 Interacted with ACTN1 and Impaired Actin Filament Aggregation into Bundles
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gu, Q.-Y.; Liu, Y.-X.; Wang, J.-L.; Huang, X.-L.; Li, R.-N.; Linghu, H. LLGL2 Inhibits Ovarian Cancer Metastasis by Regulating Cytoskeleton Remodeling via ACTN1. Cancers 2023, 15, 5880. https://doi.org/10.3390/cancers15245880
Gu Q-Y, Liu Y-X, Wang J-L, Huang X-L, Li R-N, Linghu H. LLGL2 Inhibits Ovarian Cancer Metastasis by Regulating Cytoskeleton Remodeling via ACTN1. Cancers. 2023; 15(24):5880. https://doi.org/10.3390/cancers15245880
Chicago/Turabian StyleGu, Qiu-Ying, Yue-Xi Liu, Jin-Long Wang, Xiao-Lan Huang, Ruo-Nan Li, and Hua Linghu. 2023. "LLGL2 Inhibits Ovarian Cancer Metastasis by Regulating Cytoskeleton Remodeling via ACTN1" Cancers 15, no. 24: 5880. https://doi.org/10.3390/cancers15245880