JAG1 Intracellular Domain Enhances AR Expression and Signaling and Promotes Stem-like Properties in Prostate Cancer Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Lines
2.2. Reagents and Plasmids
2.3. Cell Transfection and Reporter Assays
2.4. RNA Isolation, RT-PCR and qPCR Analysis
2.5. Western Blot Analysis
2.6. Next-Generation Sequencing (NGS) High-Throughput RNA-Seq Analysis
2.7. Generation of JICD-Overexpressing Adenovirus (AdJICD)
2.8. Development of Stable JICD-Expressing CRPC (CWR22Rv1 and C4-2) Cells
2.9. Cell Proliferation Assays
2.10. Colony Formation Assay
2.11. Cell Mobility Assays
2.12. Three-Dimensional Cell Culture Assays
2.13. Xenograft Animal Model
2.14. Quantification and Statistical Analysis
3. Results
3.1. JAG1 Regulates the Expression of AR and AR-Vs in PC Cells
3.2. JICD Upregulates the Expression of AR-Vs in PC Cells
3.3. JICD Enhances Androgen-Dependent and Androgen-Independent Transactivation of ARs
3.4. JICD Promotes Stem-Like Cell Properties in PC Cells
3.5. JICD Increases PC Cell Mobility and In Vivo Tumorigenesis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AR | androgen receptor |
ARE | androgen-response element |
AR-Vs | androgen receptor variants |
AR-FL | full-length AR |
AR-NTD-DBD | AR N-terminal and DNA-binding domain |
CE3 | cryptic exon 3 |
CRPC | castration-resistant prostate cancer |
DBD | DNA-binding domain |
LDB | ligand-binding domain |
NTD | NH2-terminal transactivation domain |
JAG1 | JAGGED1 |
JICD | JAG1 intracellular domain |
PCSC | prostate cancer stem cell |
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Tran, T.T.; Lee, K. JAG1 Intracellular Domain Enhances AR Expression and Signaling and Promotes Stem-like Properties in Prostate Cancer Cells. Cancers 2022, 14, 5714. https://doi.org/10.3390/cancers14225714
Tran TT, Lee K. JAG1 Intracellular Domain Enhances AR Expression and Signaling and Promotes Stem-like Properties in Prostate Cancer Cells. Cancers. 2022; 14(22):5714. https://doi.org/10.3390/cancers14225714
Chicago/Turabian StyleTran, Tuyen Thanh, and Keesook Lee. 2022. "JAG1 Intracellular Domain Enhances AR Expression and Signaling and Promotes Stem-like Properties in Prostate Cancer Cells" Cancers 14, no. 22: 5714. https://doi.org/10.3390/cancers14225714