Discovery of Novel Lin28 Inhibitors to Suppress Cancer Cell Stemness
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. CADD Methods
2.2. Chemicals and Protein Purifications
2.3. Fluorescence Polarization Assay
2.4. Electrophoresis Mobility Shift Assay (EMSA)
2.5. Bio-Layer Interferometry (BLI) Assays
2.6. Cell Line and Cell Maintenance and Other Routine Techniques
3. Results
3.1. Identification of a Ligand Pocket for Inhibitors to Block Lin28B and RNA Interactions
3.2. Virtual Screening to Identify Potential Inhibitors of Lin28B ZKD
3.3. Biochemical Assays to Screen and Characterize CADD Predicted Compounds
3.4. Lin28 Inhibitors Block Lin28 Functions in Cancer Cells
3.5. Lin28 Inhibitors Block Both Lin28A and Lin28B Isoforms
3.6. Lin28 Inhibitors Suppress Cancer Cell Stemness
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Radaeva, M.; Ho, C.-H.; Xie, N.; Zhang, S.; Lee, J.; Liu, L.; Lallous, N.; Cherkasov, A.; Dong, X. Discovery of Novel Lin28 Inhibitors to Suppress Cancer Cell Stemness. Cancers 2022, 14, 5687. https://doi.org/10.3390/cancers14225687
Radaeva M, Ho C-H, Xie N, Zhang S, Lee J, Liu L, Lallous N, Cherkasov A, Dong X. Discovery of Novel Lin28 Inhibitors to Suppress Cancer Cell Stemness. Cancers. 2022; 14(22):5687. https://doi.org/10.3390/cancers14225687
Chicago/Turabian StyleRadaeva, Mariia, Chia-Hao Ho, Ning Xie, Sijie Zhang, Joseph Lee, Liangliang Liu, Nada Lallous, Artem Cherkasov, and Xuesen Dong. 2022. "Discovery of Novel Lin28 Inhibitors to Suppress Cancer Cell Stemness" Cancers 14, no. 22: 5687. https://doi.org/10.3390/cancers14225687