Genetic Inactivation of Notch1 Synergizes with Loss of Trp53 to Induce Tumor Formation in the Adult Mouse Forebrain
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Tamoxifen Administration
2.3. Tissue Preparation and Immunohistochemistry
3. Results
3.1. Combined Deletion of Notch1/Notch2 and Trp53 in Hes5+ Cells Leads to the Formation of Forebrain Tumors in Adult Mice
3.2. Deletion of Notch1 and Trp53 in Hes5+ Cells Leads to the Formation of Forebrain Tumors in Adult Mice
3.3. Hyperplasia and Tumors Are Preferentially Located in Ventral Forebrain Regions and OBs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trp53-/- | Trp53-/-Rbpj-/- | Trp53-/-Notch1-/-Notch2-/- | Trp53-/-Notch1-/- | Trp53-/-Notch2-/- | |
---|---|---|---|---|---|
Hyperplasia | 49.5 ± 10.5 (n = 3) | n/a | 73.6 ± 4.3 (n = 5) | 63.7 ± 9.1 (n = 5) | 55.0 ± 3.8 (n = 2) |
Tumor | n/a | 89.6 ± 0.6 (n = 4) | 85.4 ± 4.9 (n = 4) | 76.7 ± 5.5 (n = 5) | n/a |
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Parmigiani, E.; Giachino, C. Genetic Inactivation of Notch1 Synergizes with Loss of Trp53 to Induce Tumor Formation in the Adult Mouse Forebrain. Cancers 2022, 14, 5409. https://doi.org/10.3390/cancers14215409
Parmigiani E, Giachino C. Genetic Inactivation of Notch1 Synergizes with Loss of Trp53 to Induce Tumor Formation in the Adult Mouse Forebrain. Cancers. 2022; 14(21):5409. https://doi.org/10.3390/cancers14215409
Chicago/Turabian StyleParmigiani, Elena, and Claudio Giachino. 2022. "Genetic Inactivation of Notch1 Synergizes with Loss of Trp53 to Induce Tumor Formation in the Adult Mouse Forebrain" Cancers 14, no. 21: 5409. https://doi.org/10.3390/cancers14215409