Crosstalk of Oxidative Phosphorylation-Related Subtypes, Establishment of a Prognostic Signature and Immune Infiltration Characteristics in Colorectal Adenocarcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Method
2.1. Data Acquisition
2.2. Identification of OXPHOS-Related Prognostic Signature
2.3. Functional and Gene Set Enrichment Analysis
2.4. Construction of Risk Score Signature
2.5. Cell Culture
2.6. Quantitative RT-PCR
2.7. Tumor Microenvironment (TME) Analysis
2.8. Mutational Analysis
2.9. Immunophenoscore (IPS) and Chemotherapy Analysis
2.10. Statistical Analysis
3. Results
3.1. Differentially Expressed Oxidative Phosphorylation-Related Genes
3.2. Prediction Signature Construction
3.3. OXPHOS-Related Prognostic Signature Validation
3.4. Prediction Value of OXPHOS-Related Prognostic Signature
3.5. GESA, ESTIMATE and ssGSEA Analysis
3.6. Tumor-Infiltrating Immune Cells (TIICs) Analysis
3.7. Somatic Variation Analysis
3.8. Immunotherapy Efficacy Analysis
3.9. Chemotherapy Sensitivity Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | HR | Low 95%CI | High 95%CI | p-Value |
---|---|---|---|---|
MRPS23 | 0.499 | 0.255 | 0.976 | 0.042 |
PPARGC1A | 0.546 | 0.307 | 0.972 | 0.040 |
SHH | 0.595 | 0.365 | 0.972 | 0.038 |
FSCN1 | 1.346 | 1.052 | 1.721 | 0.018 |
TRAP1 | 0.437 | 0.205 | 0.931 | 0.032 |
MPC1 | 0.433 | 0.214 | 0.878 | 0.020 |
PPRC1 | 2.288 | 1.098 | 4.766 | 0.027 |
PPA1 | 0.516 | 0.266 | 1.000 | 0.050 |
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Wang, C.; Cui, G.; Wang, D.; Wang, M.; Chen, Q.; Wang, Y.; Lu, M.; Tang, X.; Yang, B. Crosstalk of Oxidative Phosphorylation-Related Subtypes, Establishment of a Prognostic Signature and Immune Infiltration Characteristics in Colorectal Adenocarcinoma. Cancers 2022, 14, 4503. https://doi.org/10.3390/cancers14184503
Wang C, Cui G, Wang D, Wang M, Chen Q, Wang Y, Lu M, Tang X, Yang B. Crosstalk of Oxidative Phosphorylation-Related Subtypes, Establishment of a Prognostic Signature and Immune Infiltration Characteristics in Colorectal Adenocarcinoma. Cancers. 2022; 14(18):4503. https://doi.org/10.3390/cancers14184503
Chicago/Turabian StyleWang, Can, Guoliang Cui, Dan Wang, Min Wang, Qi Chen, Yunshan Wang, Mengjie Lu, Xinyi Tang, and Bolin Yang. 2022. "Crosstalk of Oxidative Phosphorylation-Related Subtypes, Establishment of a Prognostic Signature and Immune Infiltration Characteristics in Colorectal Adenocarcinoma" Cancers 14, no. 18: 4503. https://doi.org/10.3390/cancers14184503
APA StyleWang, C., Cui, G., Wang, D., Wang, M., Chen, Q., Wang, Y., Lu, M., Tang, X., & Yang, B. (2022). Crosstalk of Oxidative Phosphorylation-Related Subtypes, Establishment of a Prognostic Signature and Immune Infiltration Characteristics in Colorectal Adenocarcinoma. Cancers, 14(18), 4503. https://doi.org/10.3390/cancers14184503