Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Patients and Samples
2.3. Data Collection
2.4. Specimen Processing (DNA Extraction, Bisulfite Conversion, and Quantitative Methylation-Specific PCR)
2.5. The Frequency of Diagnosing EC among IVPs from the Taiwan Cancer Registry Database
2.6. Statistical Analysis
3. Results
3.1. Baseline Characteristics
3.2. DNA Methylation Levels and Diagnostic Performance
Where: ϵ = −5.18
3.3. MPap and Endometrial Thickness
3.4. MPap in Algorism
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cohort | Normal | Benign | Precancerous | EC | p-Value | |
---|---|---|---|---|---|---|
Stage 1 | n | 138 | 45 | 24 | 42 | |
Age, mean (SD) | 49.3 (7.2) | 51.0 (9.2) | 48.0 (6.2) | 56.7 (8.5) | <0.0001 | |
BMI, mean (SD) | 24.0 (4.2) | 25.1 (5.4) | 25.6 (5.8) | 25.7 (4.8) | 0.0966 | |
ET, mean (SD) | 11.6 (9.2) | 13.8 (6.9) | 11.8 (7.0) | 16.4 (8.8) | 0.0223 | |
BHLHE22 (Ct) | 49.7 (1.8) | 49.8 (1.4) | 49.6 (2.2) | 45.6 (6.4) | <0.0001 | |
CDO1 (Ct) | 46.0 (5.0) | 46.2 (4.2) | 45.2 (5.4) | 38.4 (5.1) | <0.0001 | |
Stage 2 | n | 86 | 83 | 37 | 39 | |
Age, mean (SD) | 49.4 (6.7) | 49.0 (6.9) | 48.3 (5.0) | 59.9 (11.0) | <0.0001 | |
BMI, mean (SD) | 24.1 (4.6) | 24.0 (3.7) | 24.1 (3.6) | 27.1 (6.2) | 0.0019 | |
ET, mean (SD) | 11.4 (13.6) | 13.8 (18.6) | 11.7 (4.7) | 16.4 (8.1) | 0.2957 | |
BHLHE22 (Ct) | 50.0 (0.0) | 49.9 (1.4) | 50.0 (0.0) | 46.1 (6.2) | <0.0001 | |
CDO1 (Ct) | 48.0 (3.4) | 47.6 (4.1) | 47.8 (3.6) | 42.4 (6.2) | <0.0001 |
Composition of Different Combined Variables | AUC (95% CI) |
---|---|
BHLHE22 + CDO1 | 0.86 (0.82 to 0.90) |
BHLHE22 + CDO1 + Age | 0.89 (0.84 to 0.92) |
BHLHE22 + CDO1 + BMI | 0.88 (0.83 to 0.92) |
BHLHE22 + CDO1 + Age + BMI | 0.91 (0.87 to 0.94) |
MPap-Value | TVUS | p-Value | ||
---|---|---|---|---|
≥Cutoff Threshold | ET | |||
Stage 1 | Sen | 92.9 (80.5–98.5) | 50.0 (32.9–67.1) | <0.0001 |
Spe | 71.5 (64.8–77.5) | 77.7 (71.2–83.3) | 0.1190 | |
PPV | 39.8 (34.4–45.5) | 29.0 (21.1–38.3) | 0.0128 | |
NPV | 98.0 (94.3–99.3) | 89.5 (85.9–92.2) | 0.0001 | |
Stage 2 | Sen | 92.5 (82.9–100.0) | 55.6 (38.1–72.1) | <0.0001 |
Spe | 73.8 (67.6–79.4) | 82.2 (76.2–87.3) | 0.0271 | |
PPV | 40.2 (30.8–50.5) | 36.4 (27.3–46.5) | 0.3936 | |
NPV | 98.1 (95.8–100.0) | 91.0 (87.5–93.6) | 0.0006 |
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Wen, K.-C.; Huang, R.-L.; Chen, L.-Y.; Wu, T.-I.; Lu, C.-H.; Chu, T.-Y.; Ou, Y.-C.; Wu, C.-H.; Hsu, S.-T.; Ding, D.-C.; et al. Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study. Cancers 2022, 14, 4343. https://doi.org/10.3390/cancers14174343
Wen K-C, Huang R-L, Chen L-Y, Wu T-I, Lu C-H, Chu T-Y, Ou Y-C, Wu C-H, Hsu S-T, Ding D-C, et al. Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study. Cancers. 2022; 14(17):4343. https://doi.org/10.3390/cancers14174343
Chicago/Turabian StyleWen, Kuo-Chang, Rui-Lan Huang, Lin-Yu Chen, Tzu-I Wu, Chien-Hsing Lu, Tang-Yuan Chu, Yu-Che Ou, Chen-Hsuan Wu, Shih-Tien Hsu, Dah-Ching Ding, and et al. 2022. "Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study" Cancers 14, no. 17: 4343. https://doi.org/10.3390/cancers14174343
APA StyleWen, K. -C., Huang, R. -L., Chen, L. -Y., Wu, T. -I., Lu, C. -H., Chu, T. -Y., Ou, Y. -C., Wu, C. -H., Hsu, S. -T., Ding, D. -C., Chu, L. -H., Chen, C. -W., Chang, H. -C., Liu, Y. -S., Wang, H. -C., Weng, Y. -C., Su, P. -H., Lin, H., & Lai, H. -C. (2022). Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study. Cancers, 14(17), 4343. https://doi.org/10.3390/cancers14174343