Molecular Profiles of Serum-Derived Extracellular Vesicles in High-Grade Serous Ovarian Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patient Sample Collection
2.2. Medium and Large EV Isolation from Serum
2.3. Medium and Large EV Characterization and Quantification
2.4. Nucleic Acid Isolation from m/lEVs
2.5. Whole-Genome Sequencing
2.6. Mutation Calling and CNV Identification
2.7. RNA Sequencing
2.8. Statistical Analysis
3. Results
3.1. Patient Groups and Sequencing Data
3.2. Somatic Alterations in EV-DNA
3.3. Comparison between EV-DNA and Tumor DNA
3.4. Transcriptomic Expression Profiles in EV-RNA
3.5. Differential Expression and Enriched Pathways between the R0 and NACT Groups
3.6. Differential Expression and Enriched Pathways between the NACT-ER and NACT-PR
Groups
3.7. Comparison between EV-RNA and Tumor RNA
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, L.; Corvigno, S.; Ma, S.; Celestino, J.; Fleming, N.D.; Hajek, R.A.; Lankenau Ahumada, A.; Jennings, N.B.; Thompson, E.J.; Tang, H.; et al. Molecular Profiles of Serum-Derived Extracellular Vesicles in High-Grade Serous Ovarian Cancer. Cancers 2022, 14, 3589. https://doi.org/10.3390/cancers14153589
Zhao L, Corvigno S, Ma S, Celestino J, Fleming ND, Hajek RA, Lankenau Ahumada A, Jennings NB, Thompson EJ, Tang H, et al. Molecular Profiles of Serum-Derived Extracellular Vesicles in High-Grade Serous Ovarian Cancer. Cancers. 2022; 14(15):3589. https://doi.org/10.3390/cancers14153589
Chicago/Turabian StyleZhao, Li, Sara Corvigno, Shaolin Ma, Joseph Celestino, Nicole D. Fleming, Richard A. Hajek, Adrian Lankenau Ahumada, Nicholas B. Jennings, Erika J. Thompson, Hongli Tang, and et al. 2022. "Molecular Profiles of Serum-Derived Extracellular Vesicles in High-Grade Serous Ovarian Cancer" Cancers 14, no. 15: 3589. https://doi.org/10.3390/cancers14153589