Impact of Anti-Angiogenic Treatment on Bone Vascularization in a Murine Model of Breast Cancer Bone Metastasis Using Synchrotron Radiation Micro-CT
Abstract
:Simple Summary
Abstract
1. Introduction
2. Methods
2.1. Sample Preparation
2.2. Synchrotron Radiation Microcomputed Tomography (SR-µCT)
2.3. Image Pre-Processing
2.4. Image Segmentation
2.5. Enhancement of the Vascular Network
2.6. Quantitative Parameters
2.6.1. Volume Fraction
- Bone volume (BV): the entire bone volume (cortex + trabeculae).
- Vessel volume (VV)
- Total volume (TV): volume inside the outer contour of the cortical bone.
- Trabecular bone volume (Tb.BV)
- Marrow cavity volume (Mar.V)
2.6.2. Local Thickness
2.6.3. Fractal Dimension
2.6.4. Structure Model Index
2.6.5. Connectivity Density
2.7. Statistical Analysis
3. Results
3.1. 3D Displays of the Segmented Bone and Blood Vessels
3.2. 3D-Measurements of Entire Bone and Blood Vasculature
3.3. 3D-Measurements of the Trabecular Bone in Tibial Metaphysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatments | Placebo | Bevacizumab | Vatalanib | Combination (Bevacizumab + Vatalanib) | |
---|---|---|---|---|---|
Time Point (T) Number of Mice (n) | |||||
T1 (day 8 after tumor cell injection) n | T1P 10 | T1B 14 | T1V 10 | T1C 11 | |
T2 (day 22 after tumor cell injection) n | T2P 7 | T2B 7 | T2V 7 | T2C 7 |
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Xu, H.; Lafage-Proust, M.-H.; Bouazza, L.; Geraci, S.; Clezardin, P.; Roche, B.; Peyrin, F.; Langer, M. Impact of Anti-Angiogenic Treatment on Bone Vascularization in a Murine Model of Breast Cancer Bone Metastasis Using Synchrotron Radiation Micro-CT. Cancers 2022, 14, 3443. https://doi.org/10.3390/cancers14143443
Xu H, Lafage-Proust M-H, Bouazza L, Geraci S, Clezardin P, Roche B, Peyrin F, Langer M. Impact of Anti-Angiogenic Treatment on Bone Vascularization in a Murine Model of Breast Cancer Bone Metastasis Using Synchrotron Radiation Micro-CT. Cancers. 2022; 14(14):3443. https://doi.org/10.3390/cancers14143443
Chicago/Turabian StyleXu, Hao, Marie-Hélène Lafage-Proust, Lamia Bouazza, Sandra Geraci, Philippe Clezardin, Bernard Roche, Françoise Peyrin, and Max Langer. 2022. "Impact of Anti-Angiogenic Treatment on Bone Vascularization in a Murine Model of Breast Cancer Bone Metastasis Using Synchrotron Radiation Micro-CT" Cancers 14, no. 14: 3443. https://doi.org/10.3390/cancers14143443